凝血酶-壳聚糖海藻酸钠微球止血作用研究  

Hemostatic Effect of Thrombin-Chitosan Sodium Loaded Alginate Microspheres

作　　者：王强[1] 郎轶咏[1] 李峥[1] 戴华[1] 

机构地区：[1]解放军第202医院药剂科,沈阳110003

出　　处：《医药导报》2012年第5期578-580,共3页Herald of Medicine

摘　　要：目的考察凝血酶-壳聚糖海藻酸钠微球止血效果。方法以壳聚糖、海藻酸钠为辅料,凝血酶为模型药物,乳化胶联法制备止血微球;建立大鼠股动脉创伤出血模型、大鼠背部渗血模型和小鼠断尾出血模型,以云南白药、创可贴、棉球止血为参比,记录止血时间,计算出血量,考察凝血酶-壳聚糖海藻酸钠微球止血效果。结果高、中、低剂量凝血酶-壳聚糖海藻酸钠微球均能明显缩短大鼠股动脉出血时间(P<0.01),减少出血量(P<0.05),止血强度随给药剂量增大而增强,无明显量效关系;高、中、低剂量凝血酶-壳聚糖海藻酸钠微球均能明显缩短小鼠断尾出血时间(P<0.01),高剂量组止血效果较明显,止血效果呈一定量效关系;高、中、低剂量凝血酶-壳聚糖海藻酸钠微球均能明显缩短大鼠背部渗血出血时间(P<0.05),降低出血量(P<0.01),高剂量缩短出血时间最迅速,止血效果明显,有一定的量效关系。结论凝血酶-壳聚糖海藻酸钠微球止血效果明显。Objective To investigate the hemostatic effect of alginate microspheres with thrombin-chitosan sodium.Methods The hemostatic microspheres were prepared by emulsion-crosslinking method,with chitosan sodium and alginate as excipients,thrombin as model drug.The models of trauma caused femoral artery bleeding in rats,blood oozing from the back of rats and bleeding from broken tails of mice were created,the model animals were treated with thrombin microspheres and compared with Yunnan Baiyao and tampons.The hemostatic time and bleeding volume were recorded to assess the hemostatic effect of thrombin microspheres.Results The thrombin microspheres at a high,middle and low dose obviously shortened hemorrhage time（P0.01）and reduced bleeding volume（P0.05）of rat femoral artery.The hemostasis was enhanced with dose,but without conspicuous dose-effect relationship.The hemorrhage time（P0.01）of broken tails in mice was significantly curtailed by thrombin microspheres in a dose-dependant manner.The hemorrhage time（P0.05）was obviously shortened and bleeding volume（P0.01）was lowered by thrombin microspheres at the high,middle and low dose,especially for the high dose.Conclusion Thrombin-chitosan sodium loaded alginate microspheres have obvious hemostasis effects.

关 键 词：壳聚糖 海藻酸钠 凝血酶 止血 

分 类 号：R973.2[医药卫生—药品] R965[医药卫生—药学]