不同产地虎杖中白藜芦醇与白藜芦醇苷含量测定  

Determination of Resveratrol and Polydatin in Polygonum cuspidatum from Different Habitats

作　　者：周雅琴[1] 樊溪源[1] 周小雷[1] 王硕[1] 袁经权[1,2] 张蓉婷[1] 韦范[1] 缪剑华[1] 

机构地区：[1]广西药用植物研究所姚新生院士重点实验室,南宁530023 [2]暨南大学中药及天然药物研究所,广州510632

出　　处：《医药导报》2012年第5期632-634,共3页Herald of Medicine

基　　金：大瑶山特色资源技术集成与产业化(桂科产0815010-7F资助课题)

摘　　要：目的建立测定广西不同产地虎杖药材中白藜芦醇和白藜芦醇苷含量的高效液相色谱法。方法色谱柱:迪马C18(4.6 mm×250 mm,5μm);流动相A:乙腈;流动相B:0.3%磷酸;梯度洗脱(15%～40%A,0～25 min);检测波长:254 nm;柱温:25℃;流速:1.0 mL.min-1。结果测得广西产虎杖药材中白藜芦醇和白藜芦醇苷最高含量分别为1.998 3%和2.776 1%,最低含量分别为0.356 1%和0.370 5%。结论所建立的方法简便可靠,快速,重复性好,结果稳定,便于对虎杖药材进行质量控制。不同产地虎杖药材中白藜芦醇和白藜芦醇苷含量差异明显。Objective To create a method for content determination of resveratrol and polydatin in Polygonum cuspidatum from different habitats of Guangxi by HPLC.Methods The determination of resveratrol and polydatin was performed with HPLC by using a C18（4.6 mm×250 mm,5 μm）column.Acetonitrile and 0.3% phosphoric acid were used as mobile phase,which flowing at 1.0 mL·min-1（with the gradient elution at15%-40%,0 to 25 min）.The detection wavelength was 254 nm,and column temperature was at 25 ℃.Results The maximum content of resveratrol and polydatin in Polygonum cuspidatum of Guangxi was 1.998 3% and 2.776 1%,respectively,and the minimum one of which was 0.356 1% and 0.370 5%,respectively.Conclusion The established HPLC method is simple,reliable,fast,repeatable and stable,which is suitable for evaluating the quality of Polygonum cuspidatum.There is great difference between Polygonum cuspidatum from different habitats of Guangxi due to various factors.

关 键 词：虎杖 白藜芦醇 白藜芦醇苷 色谱法 高效液相 产地 

分 类 号：R286[医药卫生—中药学] R927.1[医药卫生—中医学]