不同基本培养基和外植体对剑麻愈伤组织诱导及分化的影响  

Effects of Different Explants and Basic Medium on Callus Induction and Differentiation of Agave sisalana

作　　者：杨峰[1,2] 刘巧莲[2] 代真真[1,2] 陈河龙[2] 高建明[2] 郑金龙[3] 易克贤[2,3] 

机构地区：[1]海南大学农学院,海南海口570228 [2]中国热带农业科学院热带生物技术研究所 农业部热带作物生物技术重点开放实验室,海南海口571101 [3]中国热带农业科学院环境与植物保护研究所 农业部热带作物有害生物综合治理重点实验室,海南海口571101

出　　处：《热带作物学报》2012年第3期475-478,共4页Chinese Journal of Tropical Crops

基　　金：中央级公益性科研院所基本科研业务费专项(No.ITBB110213);“948”项目(No.2010-Z6);国家麻类产业技术体系建设专项资金(No.CARS-19)

摘　　要：研究4种基本培养基和3种剑麻外植体对愈伤组织诱导及分化的影响。结果表明,SH基本培养基对剑麻愈伤组织的诱导和分化效果最好,出愈率为100%,愈伤量大,分化率为21.1%;用于剑麻愈伤组织诱导的最佳外植体是剑麻叶片,其出愈率最高,愈伤量最大。在整个诱导和分化过程中,剑麻对N的需求不大,而低铵盐含量对愈伤组织的诱导和分化有利;在含有较高浓度的盐酸硫胺素(VB1)的培养基中,愈伤组织的诱导率、愈伤量和分化率都有显著提高;Cu、Mo、Co等微量元素和有机成分肌醇,对剑麻愈伤组织的诱导并非必需,但对愈伤组织的分化可能是极有利的。The effects of four kinds of basic media and three kinds of sisal explants on the callus induction and differentiation of Agave sisalana were studied. The results showed that SH had the best effect on induction and differentiation of callus. The ratio of callus was almost 100%, the weight of callus was very large and the ratio of differentiation is 21.1%. Sisal leaves had the best effect on induction of callus with the best ratio and the largest weight of callus. Sisal had low demand for nitrogen throughout the process of callus induction and differentiation, and lower concentration of ammonium salt was advantageous to induction and differentiation of callus. Higher concentration of hydrochloric acid thiamine （vitamin BI） in the media increased callus induction and differentiation significantly. It seemed that Cu, Mo, Co and inositol were not essential to the induction of sisal callus, and they may be very favorable for callus differentiation.

关 键 词：基本培养基 外植体 剑麻 愈伤组织 诱导 分化 

分 类 号：Q813.1[生物学—生物工程] S563[农业科学—作物学]