银二氧化硅核壳增强异硫氰酸荧光素酯滤纸基质室温磷光法检测赖氨酸  

Determination of Lysine by Ag@SiO_2 Nanoparticles Enhanced Room Temperature Phosphorescence of Fluorescein Isothiocyanate on Filter Paper Substrate

作　　者：杨燕[1] 朱亚先[1] 张勇[2] 

机构地区：[1]厦门大学化学化工学院化学系,厦门361005 [2]近海海洋环境科学国家重点实验室,厦门大学环境科学研究中心,厦门361005

出　　处：《分析化学》2012年第5期735-739,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(Nos.21075102;21177102);教育部博士点基金SRFDP(No.200803840015)资助项目

摘　　要：银二氧化硅核壳(Ag@SiO2)可使异硫氰酸荧光素酯(FITC)产生金属增强室温磷光(MERTP)。当所研究体系加入赖氨酸时,其MERTP猝灭。据此,建立了检测赖氨酸的新方法。在FITC和Ag@SiO2的加入量之比为1∶1.5,pH=8.0时,本方法检测赖氨酸的检出限为0.270μmol/L;线性范围为1.50～90.5μmol/L,人工合成样品加标回收率为97.0%～108.0%;相对标准偏差为2.1%(n=9)。本方法用于检测赖氨葡锌颗粒和赖氨酸磷酸氢钙片中的赖氨酸,结果满意。Metal enhanced room temperature phosphorescence（MERTP） of fluorescein isothiocyanate isomer I（FITC） was observed with the presence of proper content of Ag@SiO2 core-shell（Ag@SiO2）.The FITC-MERTP was quenched by the existing of lysine in the system.Based on this phenomenon,a novel method for determination of lysine had hereby been developed.Under the added amount ratio of FITC to Ag@SiO2 of 1∶1.5,pH of 8.0,the detection limit for lysine was 0.270 μmol/L,the response was linearly proportional to the concentration of lysine in the range of 1.50-90.5 μmol/L,the recoveries of standard addition of artificial samples were in the range of 97.0%-108.0%,the relative standard deviation was less than 2.1%（n=9）.A satisfactory result was obtained when the established method applied to the determination of lysine in compound lysine hydrochloride and zinc gluconate granules and lysine hydrochloride and calcium hydrogen phosphate tablets

关 键 词：赖氨酸 银二氧化硅核壳 异硫氰酸荧光素酯 室温磷光 

分 类 号：O657.3[理学—分析化学]