农杆菌介导的ACO基因RNAi载体转化百合抗衰老研究  

Agrobacterium-mediated transformation of RNAi vector with ACO gene on anti-aging of lily

作　　者：李小玲[1] 刘雅莉[2] 华智锐[1] 

机构地区：[1]商洛学院生物医药工程系,陕西商洛726000 [2]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《干旱地区农业研究》2012年第2期88-92,共5页Agricultural Research in the Arid Areas

基　　金：国家自然科学基金项目(30371013);陕西省科技厅项目(2009K01-11)

摘　　要：本研究旨在通过根癌农杆菌介导法将ACO基因转入亚洲百合植株中,为植物抗衰老提供理论依据和技术基础。以亚洲百合‘精粹’无菌苗离体小鳞片为外植体,利用农杆菌介导法将携带有抗衰老基因ACO的RNAi载体转化到百合中,观测分析影响百合遗传转化的因素。结果表明:在遗传转化过程中,预培养3 d后,在菌液和共培养基中均添加25 mg/L乙酰丁香酮,将OD600=0.8的根癌农杆菌侵染小鳞片8 min,再共培养3 d,有利于获得最高遗传转化效率,抗性植株经PCR检测部分呈现阳性,初步证明ACO基因已经导入到百合基因组中。With the scale of asiatic lily as explants,the RNAi vector with ACO anti-aging gene was transferred into lily with Agrobacterium-mediated transformation to study the factors which affected the transformation and to provide theoretical and technical basis for anti-aging of plants.The results showed that the highest transformation efficiency was obtained through the following transformation procedure： after preculture for 3 d,25 mg/L acetosyringone（AS） was added to Agrobacterium and co-cultivation medium respectively,the explants were inoculated for 8min with diluted OD600=0.8 recombinant Agrobacterium tumefacien culture liquid,and then co-cultivated for 3 d.PCR detection showed that some resistant plants were positive,which indicated preliminarily that the ACO gene was integrated into the genome of transgenic plants.

关 键 词：农杆菌介导 ACO基因 RNAi表达载体 抗衰老 百合 

分 类 号：Q789[生物学—分子生物学]