高效液相色谱法测定烟草料液中苯甲酸的不确定度  

Uncertainty in Determination of Benzoic Acid in Tobacco Casing by HPLC

作　　者：钟秋瓒 黎茶根 刘小平 

机构地区：[1]赣州市烟草科学研究所 [2]江西省烟草公司,南昌市洪城路298号330025

出　　处：《烟草科技》2012年第5期31-34,共4页Tobacco Science & Technology

摘　　要：为提高检测结果的准确性,依据JJF 1059—1999《测量不确定度评定与表示》系统计算了高效液相色谱(HPLC)法测定烟草料液中苯甲酸含量的不确定度。结果表明:①拟合工作曲线和回收率对测量不确定度影响最大;②当苯甲酸含量为6.98 mg/kg,其合成不确定度为0.23 mg/kg,扩展不确定度为0.46 mg/kg(置信度95%,k=2)。采用高纯度标准品和高精度的容器,严格控制处理步骤,可以降低HPLC法测定烟草料液中苯甲酸的不确定度。In order to improve the accuracy of test results, the uncertainty in the determination of benzoic acid in tobacco casing by HPLC was systematically calculated according to JJF 1059--1999 Evaluation and Expression of Uncertainty in Measurement. The results indicated that： 1） calibration curve and recovery of benzoic acid were the main factors influencing the uncertainty; 2） the combined uncertainty was 0.23 mg/kg and the expanded uncertainty was 0.46 mg/kg （level of confidence 95%, k = 2） for the tobacco casing contained 6.98 mg/kg of benzoic acid. It was concluded that the uncertainty in determination of benzoic acid in tobacco casing by HPLC could be reduced by using high purity standard, high precision containers, and strictly controlling of preparation procedures.

关 键 词：不确定度 HPLC 苯甲酸 烟草料液 

分 类 号：TQ656.5[化学工程—精细化工]