采用全烟气暴露系统测试卷烟主流烟气细胞毒性  

In vitro Cytotoxicity Assay for Mainstream Cigarette Smoke with Whole Smoke Exposure System

作　　者：李翔[1] 聂聪[1] 尚平平[1] 谢复炜[1] 刘惠民[1] 谢剑平[1] 

机构地区：[1]中国烟草总公司郑州烟草研究院

出　　处：《烟草科技》2012年第5期44-47,共4页Tobacco Science & Technology

基　　金：国家自然科学基金资助项目"卷烟主流烟气和侧流烟气中颗粒度的尺度;化学组成与生物毒性研究"(21007094);国家烟草专卖局科技项目"卷烟危害性指标体系深化研究"(110200801022);国家烟草专卖局标准项目"卷烟全烟气暴露的体外毒理学测试方法"(2011B056)

摘　　要：基于VITROCELL系统结合中性红摄取试验建立了全烟气暴露细胞毒性测试方法,采用建立的方法测试了加拿大深度(HCI)抽吸条件下国内外29个牌号市售卷烟主流烟气的细胞毒性。结果表明:①建立的全烟气暴露细胞毒性测试方法可以获得良好的烟气细胞毒性剂量-效应关系;②测试结果的变异系数(CV)在25%以内,方法稳定性较好。建立的全烟气暴露实验方法适合于卷烟主流烟气细胞毒性测试。The method of in vitro cytotoxicity assay for mainstream cigarette smoke was established by using VITROCELL whole smoke exposure system associated with neutral red uptake assay. The cytotoxicity of mainstream cigarette smoke of 29 domestic and imported commercial available brands was evaluated by this method under Health Canada Intensive （HCI） smoking regime. The results indicated that： 1） A well dose-response relationship between cigarette smoke doses and cell viability could be obtained by the established method. 2）The stability of the method was satisfactory with the values of CV below 25%. The established method of whole smoke exposure could be applied to evaluate the cytotoxicity of mainstream cigarette smoke.

关 键 词：卷烟烟气 全烟气 HCI抽吸条件 中性红 细胞毒性 

分 类 号：TS416[农业科学—烟草工业]