烟碱降解细菌528菌株的分离鉴定和降解特性  

Isolation,Identification and Characteristics of Nicotine-degrading Bacterium Strain 5-28

作　　者：陈晨[1,2] 麻广慧[2] 雷丽萍[3] 周薇[2] 沈兴家[1] 杨金奎[2] 

机构地区：[1]江苏科技大学生物与化学工程学院,江苏省镇江市四摆渡212018 [2]云南大学生物资源保护与利用重点实验室,昆明市翠湖北路2号650091 [3]云南省烟草农业科学研究院,云南省玉溪市南祥路14号653100

出　　处：《烟草科技》2012年第5期74-78,共5页Tobacco Science & Technology

基　　金：国家自然科学基金面上项目"红球菌Y22菌株降解尼古丁的代谢途径和分子基础研究"(31060012);中国烟草总公司云南省公司科技项目"降烟碱微生物菌剂及应用技术研究"(20010YN16)

摘　　要：为解决我国一些烟区烤烟烟碱含量偏高问题,筛选了具有降解烟叶烟碱含量应用潜力的细菌。以烟碱为唯一的碳氮源,从云南省弥勒县的烟草栽培土壤中分离得到1株烟碱降解细菌5-28。经形态学观察和生理生化性质分析,结合16S rRNA测序和系统进化分析将该细菌鉴定为根瘤菌(Sinorhizobiumsp.)5-28。该菌能耐受2.5 g/L的烟碱,对烟碱的最适降解条件为:烟碱浓度1.5 g/L,pH 4.0,温度25℃。经过24 h培养,菌株5-28对初始浓度为1 g/L的烟碱降解率接近80%,同时,该菌的休止细胞也能有效降解烟叶中的烟碱。In order to solve the problem of higher nicotine content in flue-cured tobacco leaves produced in some areas, bacterium strain 5-28, which had the potential of degrading nicotine was isolated from tobacco growing soil in Mile, Yunnan using nicotine as the sole source of carbon and nitrogen. The strain 5-28 was identified as Sinorhizobium sp. 5-28 via morphological observation, physio-biochemical property analysis, 16S rRNA sequencing and phylogenetic analysis. The strain 5-28 is tolerant to nicotine at a concentration of 0-2.5 g/L. The optimum condition for nicotine degradation is： nicotine concentration 1.5 g/L, pH 4.0 and 25 ℃. Almost 80% nicotine would be degraded after cultivation for 24 hours where the initial concentration was I g/L, moreover, the resting cells of strain 5-28 would effectively degrade the nicotine in tobacco leaf as well.

关 键 词：烟碱 根瘤菌 分离鉴定 生物降解 

分 类 号：TS414[农业科学—烟草工业]