Study on SSR Markers Linked to Flesh Color around the Stone of Prunus persica(L.) Batsch  

SSR方法标记桃[Prunus persic(L.)Batsch]果肉近核色素(英文)

作　　者：韩继成[1,2] 刘国俭[2] 常瑞峰[2] 张新忠[1] 

机构地区：[1]东北农业大学园艺学院,黑龙江哈尔滨150051 [2]河北省农林科学院昌黎果树研究所,河北昌黎066600

出　　处：《Agricultural Science & Technology》2012年第5期962-964,共3页农业科学与技术（英文版）

基　　金：Supported by Fund of Hebei Academy of Agriculture and Forestry Sciences(A06120203)~~

摘　　要：[Objective] This study aimed to select SSR molecular markers linked to flesh color around the stone of Prunus persica （L.） Batsch. [Method] P. persica （L.） Batsch varieties Chongyanghong and Yanhong were used as parents to construct F1 orthogonal group. A total of 138 FI individuals were selected as experimental materi- als for construction of color around the stone gene pool （B1） and non-color around the stone gene pool （B2） by using bulked segregant analysis （BSA） method, molec- ular markers linked to the flesh color around the stone of P. persica （L.） Batsch were selected with SSR molecular marker technology. [Result] After selection with 256 pairs of SSR primers, three pairs of molecular markers linked to the gene con- trolling flesh color around the stone of P. persica （L.） Batsch were selected （UDP96- 003, ch04g09 and UDP97-402）. In addition, genetic distances between the three molecular markers and the gene controlling flesh color around the stone of P. persi- ca （L.） Batsch were calculated, which were 16.7, 10.1 and 17.0 cM, respectively. [Conclusion] This study laid the foundation for further selection of co-dominant molecular markers with closer genetic distance.[目的]利用 SSR 分子标记法标记桃(Prunus persic (L.) Batsch)果肉近核色素。[方法]以"重阳红"与"燕红"2 个桃品种为亲本构建正交 F1群体,选取其中138株后代作为标记群体,采用分离群体分组分析(bulked segregate analysis,BSA)法,将果肉近核色素分为"有"和"无"2个基因池,应用SSR分子标记技术寻找与桃果肉近核色素性状基因连锁的分子标记。[结果]通过对256对引物的筛选,获得了3对与控制桃果肉近核色素性状基因连锁的分子标记,即 UDP96-003、ch04g09 和 UDP97-402,同时计算得到这 3 个标记与桃果肉近核色素性状基因的遗传距离分别为16.7、10.1和17.0 cM。[结论]该研究为进一步筛选遗传距离更近的共显性分子标记奠定了基础。

关 键 词：Prunus persica （L.） Batsch Flesh Color around the stone SSR 

分 类 号：S662.1[农业科学—果树学]