cDNA Cloning and Sequence Analysis of ADH Gene in Delia antiqua  

葱蝇ADH基因的克隆及序列分析(英文)

作　　者：陈春露[1] 陈斌[1] 司风玲[1] 何正波[1] 

机构地区：[1]重庆师范大学昆虫与分子生物学研究所,重庆高校生物活性物质工程研究中心,重庆市动物生物学重点实验室

出　　处：《Agricultural Science & Technology》2012年第5期1007-1010,共4页农业科学与技术（英文版）

基　　金：Supported by National Natural Science Foundation of China (30870340,31071968);Scientific and Technological Research Project of Chongqing Municipal Education Commission (KJ100620);Key Project of Chongqing Normal University (2011XLZ12)~~

摘　　要：[Objective] This study aims to conduct cloning and sequence analysis of ADH gene in D. Antiqua. [Method] Full-length cDNA of ADH gene in D. antiqua was cloned by using RACE technology （GenBank access number： JQ666006）. Analysis of the homology, characteristics and functional domains of ADH sequence and the phy- Iogenetic relationship to other dipteran ADH were conducted. [Result] The full length of ADH cDNA is 1 088 bp containing a 771 bp of ORF, encoding 256 amino acids, with a calculated relative molecular weight of 30.80 kDa and a theoretical isoelectric point of 8.22. The deduced amino acid sequence shares the highest homology with Glossina morsitans morsitans based on homological analysis and phylogenetic analysis. [Conclusion] This study provides basis for further research of ADH gene.[目的]对葱蝇(Delia antiqua)ADH基因进行克隆,并对其进行序列分析。[方法]通过RACE的方法克隆葱蝇ADH基因的cDNA序列,同时对该序列进行同源性分析、氨基酸序列比对和系统发育分析。[结果]试验获得的cDNA全长1088bp,其中ORF771bp,编码256个氨基酸,推测其相对分子质量为30.80kDa,等电点为8.22;通过该基因推导的氨基酸序列与其他物种的ADH进行相似性比较和系统发育分析,发现葱蝇与刺舌蝇(Glossina morsitans morsitans)氨基酸序列的同源性最高。[结论]该研究为ADH基因的进一步研究提供了基础。

关 键 词：Delia antiqua ADH Sequence analysis Phylogenetic relationship 

分 类 号：S436.3[农业科学—农业昆虫与害虫防治]