不结球白菜谷胱甘肽还原酶基因NhccGR1的克隆与表达分析  

Cloning and expression analysis of glutathione reductase gene NhccGR1in non-heading Chinese cabbage

作　　者：李彦肖[1] 彭海涛[1] 管苇[1] 许小海[1] 李英[1] 侯喜林[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室,江苏南京210095

出　　处：《南京农业大学学报》2012年第3期7-12,共6页Journal of Nanjing Agricultural University

基　　金：现代农业产业技术体系建设专项资金项目(CARS-25-A-12);国家大学生创新性实验计划资助项目(101030708)

摘　　要：利用cDNA-AFLP技术,从芜菁花叶病毒(TuMV)侵染的不结球白菜(Brassica campestris ssp.chinensis Makino)幼叶中分离到1条编码谷胱甘肽还原酶的基因片段,通过电子克隆得到其cDNA全长为1 503 bp,编码501个氨基酸,命名为Nhc-cGR1。对该基因进行生物信息学分析,并利用实时定量PCR研究其在TuMV侵染和水杨酸(SA)、茉莉酸(JA)、乙烯(ET)诱导下的表达情况。结果表明:该基因作为病原相关基因参与了TuMV病害响应,并通过增加自身表达量来激活细胞内的信号转导途径,诱导各种防卫反应相关基因的表达;同时,SA和JA抑制该基因的表达,外施ET能诱导其正向表达。A glutathione reductase gene was isolated from Turnip mosaic virus（TuMV）-infected seedling leaves of non-heading Chinese cabbage（Brassica campestris ssp.chinensis Makino）by cDNA-AFLP technique.The gene,named NhccGR1,was 1 503 bp in size and encoded 501 amino acids by in silico cloning.Bioinformatics was performed to the gene.In addition,it was analyzed by quantitative real-time PCR to monitor its expression patterns under TuMV infection and inductions of salicylic acid（SA）,jasmonic acid（JA）and ethylene（ET）.The results indicated that the gene involved in TuMV-infection as a pathogensis gene.It led to the expression of a variety of defense response genes by increasing its own expression.At the same time,it participated in the induction of SA,JA and ET.SA and JA inhibited the gene expression,while external ET increased its expression.

关 键 词：不结球白菜 谷胱甘肽还原酶 芜菁花叶病毒(TuMV) 诱导 基因表达 

分 类 号：S634.3[农业科学—蔬菜学]