柳树中生物碱类化合物抗肿瘤活性的体外实验研究  

Experimental Study in Vitro of Anti-tumor Activity of Alkaloids in Willow

作　　者：周全[1,2] 陈建伟[1] 刘晓蓉 

机构地区：[1]南京中医药大学药学院,江苏南京210046 [2]南京圣和药业有限公司,江苏南京210018

出　　处：《南京中医药大学学报》2012年第3期238-240,共3页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：企业创新药物孵化基地建设(2011ZX09401-003)

摘　　要：目的通过从灌木Combretum Caffrum分离得到的6种生物碱类化合物进行体外抗肿瘤活性复筛选。方法采用MTT法观察这6种化合物对K562(人粒性白血病细胞),MCF-7(人乳腺癌细胞),DU-145(人前列腺癌细胞),NCI-H460(人非小细胞肺癌细胞)、HCT-116(人结肠癌细胞细胞)6种人癌细胞株的影响。结果 6个化合物具有不同程度的抑制人癌细胞株增殖的作用,其中以C002化合物体外抗肿瘤活性最强。结论 C002化合物体外抗肿瘤活性与CA4相当,有潜力成为新一代肿瘤血管阻断剂候选药物。OBJECTIVE To screen the 6 alkaloids Abstracted from Combretum Caffrum according to their anti-tumor activity.METHODS MTT was applied to observe the effect of these 6 alkaloids on K562,MCF-7,DU-145,NCI-H460 and HCT-116.RESULTS They inhibited the proliferation of human tumor cell strains to different degree,especially C002 which was of greatest anti-tumor activity.CONCLUSION C002,as effective as CA4 on anti-tumor,has the potential to become a new drug as tumor blood vessel blocker.

关 键 词：生物碱类 癌细胞株 抗肿瘤 MTT法 

分 类 号：R965.1[医药卫生—药理学]