基于“工艺-指纹图谱-效应”模式构建通塞脉微丸谱效相关性指纹图谱  

Tongsaimai Pellets Spectral Efficiency Related Fingerprint Establishment Based on "Technology-fingerprint-effect" Model Research

作　　者：陈璟[1] 王皓[1] 汪海鸿[1] 恽菲[1] 狄留庆[1] 赵晓莉[1] 蔡宝昌[1] 

机构地区：[1]南京中医药大学、南京市中药微丸产业化工程技术研究中心,江苏南京210029

出　　处：《南京中医药大学学报》2012年第3期259-264,共6页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家科技部863重大专项(2003AA2Z3247);国家自然科学基金(30873401,81073071);江苏省自然科学基金(BK2005150);江苏省“青蓝工程”科技创新团队支持计划(2008);江苏省优势学科建设工程资助项目

摘　　要：目的基于"工艺-指纹图谱-效应"研究模式探讨构建通塞脉微丸谱效相关性指纹图谱的研究。方法以通塞脉微丸为研究对象,通过不同提取分离工艺引起该中间提取物中化学成分组成的改变,进而引起指纹图谱特征信息变化和主要药效学变化,利用UPLC/MS-Q-TOF技术对通塞脉微丸不同工艺中间提取物化学成分进行了初步解析,结合药效学实验数据,运用层次分析法探讨通塞脉微丸谱效相关性,构建谱效相关性指纹图谱。结果肉桂酸、绿原酸、LS-A2、阿魏酸等20个化合物对于通塞脉微丸药效活性的贡献值较其他化学成分高。结论基于"工艺-指纹图谱-效应"模式研究构建中药复方制剂谱效相关性指纹图谱,是表征复方整体成分-药效相关性的有效途径。OBJECTIVE The study was based on ＂technology-fingerprint-effect＂ model of the Tongsaimai Pellets＇ correlation between spectral efficiency research.METHODS Tongsaimai Pellets based extraction process caused by different chemical composition of the compound changes,thereby causing changes in fingerprinting of information and pharmacodynamic changes,Experiments using UPLC/MS-Q-TOF technology Tongsaimai Pellets extract of different chemical composition of intermediate processes conducted a preliminary analysis,Combined with pharmacodynamics experiment data,Use Pearson correlation coefficient And the level analysis the Tongsaimai spectrum correlation effect,Constructing spectrum correlation effect.RESULTS Cinnamic acid,chlorogenic acid,lsdyna A2,the ferulic acid and so on for the Tongsaimai the activity of contribution value than other chemical composition high.CONCLUSION The study was based on ＂technology-fingerprint-effect ＂ Model of the correlation between spectral efficiency,which is an effective way of characterizing the correlation of Compound overall composition and pesticide effect.

关 键 词：通塞脉微丸 指纹图谱 谱效相关性 

分 类 号：R285[医药卫生—中药学]