HPLC法测定Liguzinediol原料药的含量及有关物质  

HPLC Determination of Liguzinediol and its Related Substances

作　　者：房敏[1] 李伟[1] 文红梅[1] 刘峥[1] 陈磊[1] 

机构地区：[1]南京中医药大学药学院,江苏南京210046

出　　处：《南京中医药大学学报》2012年第3期279-281,共3页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家科技部"重大新药创制"专项(2009ZX09103-081);国家自然科学基金(1072542)

摘　　要：目的建立高效液相色谱法测定Liguzinediol原料药的含量及其有关物质。方法采用Phecda C18柱(250mm×4.6mm,5μm),以甲醇-水溶液(20∶80)为流动相,流速为1.0mL/min,检测波长为278nm,柱温为30℃,进样量10μL。结果主峰能与相邻杂质峰较好分离。Liguzinediol在60～140μg/mL浓度范围内,与峰面积呈良好的线性关系,r=0.999 9。结论本法准确、方便、专属性强,可用于测定Liguzinediol原料药含量及有关物质。OBJECTIVE To establish an HPLC method to determine the content of Liguzinediol and its related substances.METHODS The assay was conducted on a Phecda C18 column（250 mm×4.6 mm,5 μm） with methanol-water（20∶80） as mobile phase at the flow rate of 1.0 mL/min and the detection wavelength was set at 278 nm.RESULTS Liguzinediol was completely separated from impurities.The linear concentration range of Liguzinediol was 60～140 μg/mL with the correlation coefficient of 0.9999.CONCLUSION This accurate and reliable HPLC method is applicable for the quality control of Liguzinediol and its related substances.

关 键 词：高效液相色谱法 LIGUZINEDIOL 含量测定 有关物质 

分 类 号：R284.1[医药卫生—中药学]