高效液相色谱法测定大败毒胶囊中大黄酚含量  

Determination of Chrysophanol in Dabaidu Capsules by HPLC

作　　者：王述蓉[1] 叶云[1] 肖顺林[1] 

机构地区：[1]泸州医学院附属医院药剂科,四川泸州646000

出　　处：《中国药业》2012年第10期30-31,共2页China Pharmaceuticals

摘　　要：目的测定大败毒胶囊中大黄酚的含量。方法采用高效液相色谱法,Scienhome C18色谱柱(150 mm×4.6 mm,5μm),流动相为甲醇-0.1%磷酸(80∶20),流速为1.0 mL/min,检测波长为254 nm,柱温为30℃。结果大黄酚进样量在0.04～1.00μg范围内与峰面积的线性回归方程为Y=9.671 X+3.258,r=0.999 8,平均回收率为98.19%,RSD为1.01%(n=9)。结论所建立的方法简便、准确、重复性好,可用于大败毒胶囊中大黄酚的含量测定。Objective To establish a method for determining chrysophanol in Dabaidu Capsules. Methods The HPLC method was adopt- ed. The Scienhome C18 column（150 mm×4.6 mm, 5 μm） was used at the column temperature of 30℃.The mobile phase was methanol-0. 1% phosphoric acid （80：20） with the flow rate of 1.0 mL/min and the detection wavelength of 254 nm. Results The method displayed a good linear relationship in the range of 0.04 - 1.00 μg for chrysophanol,the regression equation was Y = 9.671 X ＋ 3.258 （r=0. 999 8）. The average recovery rate was 98. 19% with RSD of 1.01% （n=9）. Conclusion The established method is simple, accurate,repeatable and suitable for the determination of chrysophanol in Dabaidu Capsules.

关 键 词：大黄酚 大败毒胶囊 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]