正交试验优选人参多糖除蛋白工艺  

Optimization on Protein-removing Technology of Ginseng Polysaccharides by Orthogonal Test

作　　者：宋利华[1,2] 萧伟[3] 

机构地区：[1]南京中医药大学药学院,南京210000 [2]江苏康缘药业股份有限公司,连云港222001 [3]江苏康缘药业股份有限公司中药制药过程新技术国家重点实验室,连云港222001

出　　处：《世界科学技术-中医药现代化》2012年第2期1502-1507,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：科学技术部国家“973计划”项目(2010CB735604):新药研制过程化学机理,负责人:萧伟

摘　　要：目的:优选人参多糖的最佳除蛋白工艺。方法:以蛋白清除率、多糖保留率和糖醛酸保留率为指标,比较了两种不同除蛋白方法(Sevag法和TCA法)的效果,并采用正交试验优选最佳除蛋白工艺。结果:Sevag法的除蛋白效果优于TCA法,人参多糖的最佳除蛋白工艺为5%人参多糖溶液中加入1/3倍体积的Sevag试剂,V氯仿∶V正丁醇为4∶1,共除6次。结论:该工艺除蛋白效率较高,可用于人参多糖的分离精制。This study was aimed to optimize conditions for protein-removing from ginseng polysaecharides. The pro- tein-removing rate, retaining rate of polysaccharides and uronic acid were used as indicators. Results of two protein- removing methods （i.e., Sevag method and TCA method） were compared. And the optimized protein-removing condi- tions were obtained by orthogonal test. The results of Sevag method were better than TCA method. The optimized con- dition for protein-removing from ginseng polysaccharides was that Sevag reagent volume was equal to 1/3 times of 5% ginseng polysaccharides solution. And the volume ratio of CHC13 and normal butanol was 4：1. The protein-removing process was conducted 6 times. It was concluded that the method is efficient and can be used for the separation and refinement of ginseng polysaccharides.

关 键 词：人参多糖 除蛋白 正交试验 

分 类 号：R284.3[医药卫生—中药学]