正交试验优选胆木中异长春花苷内酰胺提取工艺  

Optimization of the Extraction Technology of Strictosamide from Lignum Naucleae by Orthogonal Experiment

作　　者：潘桂玲[1] 孟兆青[2,3] 刘文君[2,3] 丁岗[2,3] 原少伟[2,3] 苗宏伟[2,3] 萧伟[2,3] 

机构地区：[1]徐州医学院附属医院,徐州221002 [2]江苏中康药物科技有限公司,南京210017 [3]江苏康缘药业股份有限公司中药制药过程新技术国家重点实验室江苏省企业院士工作站,连云港222001

出　　处：《世界科学技术-中医药现代化》2012年第2期1508-1511,共4页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：科学技术部国家“重大新药创制”项目(2009ZX09103-389):候选药物-异长春苷内酰胺的研究,负责人:丁岗;江苏省科技支撑-社会发展项目(BE2009618):围绕中药胆木开展一、四类创新新药的临床前研究,负责人:丁岗

摘　　要：目的:优选胆木心材中异长春花苷内酰胺的提取工艺。方法:以异长春花苷内酰胺提取得率为评价指标,以乙醇浓度、乙醇用量、提取时间和提取次数为考察因素,通过正交试验选取最佳提取工艺。结果:最佳提取工艺为提取所用乙醇浓度为70%,乙醇加入量为药材的22倍,提取时间为4 h,提取次数为3次。结论:该工艺稳定、合理、可行,可为工业化提取胆木中异长春花苷内酰胺提供理论依据。This study was aimed to optimize the extraction technology of strictosamide from Lignum Naucleae. The extracting technology of strictosamide was optimized by orthogonal experiment with the concentration and amount of alcohol, extracting time and frequency as factors. The results showed that the optimal extraction technology was to add 22 times of alcohol to the herbal medicine at the concentration of 70%. And the extraction was continued for 4 hours and repeated for three times. It was concluded that this extraction technology is stable, reasonable and feasible. This method can provide a theoretical basis for the industrial extraction of strictosamide from Lignum Naucleae.

关 键 词：胆木 异长春花苷内酰胺 正交试验 提取工艺 

分 类 号：R284.1[医药卫生—中药学]