孕激素对大鼠阴道上皮细胞的影响  

Effect of progesterone on vaginal epithelium of ovariectomized rat

作　　者：陈雯[1] 赖爱鸾[2] 

机构地区：[1]航空总医院,北京100012 [2]首都医科大学附属复兴医院,北京100038

出　　处：《吉林医学》2012年第14期2923-2924,共2页Jilin Medical Journal

摘　　要：目的:研究孕激素对大鼠阴道上皮厚度、细胞层数以及雌激素受体、孕激素受体表达的影响。方法:本研究选用性成熟期雌性SD大鼠40只,体重285～329 g,随机分为4组,对照组(D组)、实验组1(S1组)、实验组2(S2组)、实验组3(S3组),每组10只,全部行双侧卵巢切除术。D组给予灭菌植物油肌内注射,S1组、S2组、S3组分别给予黄体酮1 mg/(kg.d)、5 mg/(kg.d)、25 mg/(kg.d)肌内注射。4周后采用放射免疫法测血清雌激素、孕激素水平,取大鼠阴道上段同一部位组织制成病理切片,测量阴道上皮厚度、细胞层数,采用免疫组织化学方法进行雌激素受体(ER)、孕激素受体(PR)的染色,计数阴道上皮细胞内细胞核ER、PR阳性的细胞所占比例。结果:大鼠血清中孕酮浓度S3组>S2组>S1组>D组,组间比较差异有统计学意义(P<0.01),上皮厚度及细胞层数S2组、S3组多于D组,组间比较差异有统计学意义(P<0.01),随着孕激素的剂量增大S1组、S2组、S3组阴道上皮的ER-、PR的阳性表达评分不断降低,ER-的阳性表达率D组显著高于S2组、S3组,组间比较差异有统计学意义(P<0.01),D组中PR的阳性表达显著高于S3组,两组比较差异有统计学意义(P<0.01)。结论:孕激素可促进卵巢切除大鼠阴道上皮厚度及细胞层数增加,并可抑制阴道上皮中雌激素、孕激素受体的表达。Objective To assess changes in conformation of vaginal epithelium when administrate different dose progesterone to ovariectomized rats.The expression and distribution of ER（estrogen receptor） and PR（progesterone receptor） in vaginal epithelium in response to different dose progesterone administration was observed in this study.Method Female rats were ovariectomized.Ovariectomized animals were treated with vehicle,low dose progesterone[1 mg/（kg·d）],middle dose progesterone[5 mg/（kg·d）],high dose progesterone[25 mg/（kg·d）].We observed the conformation of vaginal epithelium.Using immunohistochemistry,ER and PR distribution was assessed in vaginal epithelium.Results The height of vaginal epithelium and layers of cell in vaginal epithelium of the ovariectomized rats in the group that administrated progesterone is more than that of the ovariectomized rats in the group that administrated vehicle.Giving high dose progesterone to ovariectomized rats can down-regulated ER and PR in vaginal epithelium.Conclusion Progesterone can increase the height of vaginal epithelium and layers of cell in vaginal epithelium of the ovariectomized rats,and can inhibit the expression of ER and PR in vaginal epithelium.

关 键 词：大鼠 孕激素 阴道上皮 雌激素受体 孕激素受体 

分 类 号：R965[医药卫生—药理学]