不同孔径多孔CPC材料修复兔大段骨缺损的实验研究  

Experiment study on repair of large bone defect by porous calcium phosphate cement with three different pore sizes

作　　者：赵轶男[1] 刘建[1] 刘昌盛[2] 白峰[1] 孟国林[1] 郝赋[1] 

机构地区：[1]第四军医大学西京医院全军骨科研究所,西安710032 [2]华东理工大学材料科学与工程学院生物材料研究所,上海200237

出　　处：《中国矫形外科杂志》2012年第10期924-927,I0004,共5页Orthopedic Journal of China

基　　金：国家科技支撑计划(编号:2008BAI16B02)

摘　　要：[目的]探讨孔径大小对多孔磷酸钙骨水泥(CPC)修复骨缺损能力的影响。[方法]用盐析法制备三种不同孔径(200～300μm、300～450μm、450～600μm)但孔隙率相同(68.3±3.3)%的多孔CPC材料,将材料植入兔大段桡骨缺损模型,术后4、12周进行大体观察、X线检查、血清骨源性碱性磷酸酶(BALP)检测、组织学观察和生物力学检测。[结果]术后4、12周X线检查可见200～300μm孔径材料骨缺损区愈合相对较好;4周时BALP检测200～300μm组高于其他组,差异具有统计学意义(P<0.05),12周BALP检测三组差异无统计学意义;12周组织形态计量学分析显示200～300μm材料组新生骨面积百分比最多,差异有统计学意义(P<0.05);力学结果显示200～300μm组材料的最大压缩载荷和弹性模量均好于其他两组,差异具有统计学意义(P<0.05)。[结论]孔径的大小可以影响骨生物材料在体内的成骨能力,本研究中较小孔径材料成骨能力更好,且更易达到成骨性能和力学性能的统一。[Objective]To investigate the effect of pore sizes of porous calcium phosphate cement on repair of large bone defect.[Method]Three kinds of CPC scaffolds with different pore sizes（200-300μm,300-450μm,450-600μm） but same porosity（68.3±3.3%）were manufactured by salt fractionation.We implanted the scaffolds into the rabbit large bone defect model.At 4 and 12 weeks after the operation,we took the general observation,X-ray examination,serum bone alkaline phosphatase（BALP） testing,histology and biomechanical testing.[Result]The X-ray examination showed that good bone healing occurred in the group which pore size was 200-300 μm in 4 and 12 weeks after the operation.The BALP values in 200-300 μm group were significantly higher than those in the other groups in 4 week（P0.05）,but there were no significant difference among the three groups in 12 week.Histomorphology analysis indicated that the area of new bone formation in 200-300 μm group was larger than those in the others（P0.05）.The mechanical test showed that the maximum compression load and elastic modulus in 200-300 μm group was significantly higher than those in the other groups（P0.05）.[Conclusion]Pore sizes can affect osteogenesis of scaffolds in vivo.The smaller pore sizes of scaffolds in the present study can stimulate the new bone formation.

关 键 词：孔径 骨缺损 磷酸钙骨水泥 孔隙结构 

分 类 号：R687.3[医药卫生—骨科学]