葡萄SA和JA信号转导重要基因克隆及其对外源信号应答分析  

Cloning of Several Important Genes Involved in Grapevine SA and JA Signaling Pathways and Their Response to Exogenous Signals

作　　者：王文艳[1] 岳林许[2] 张演义[3] 初建青[1] 张晓莹[1] 房经贵[1] 

机构地区：[1]南京农业大学园艺学院,南京210095 [2]山东省轻工农副原料研究所,山东高密261500 [3]聊城大学,山东聊城252059

出　　处：《园艺学报》2012年第5期817-827,共11页Acta Horticulturae Sinica

基　　金：教育部新世纪优秀人才支持计划项目(NCET-08-0796);江苏省农业科技支撑项目(BE2010326)

摘　　要：以‘藤稔’葡萄（Vitisvinifera×V.labrusca‘Fujiminori’）的叶片为试材,克隆了水杨酸（SA）和茉莉酸（JA）信号转导途径中重要基因NPR1、PR1、COI1和LOX2,利用定量和半定量PCR法研究其在SA与JA处理后的表达情况,发现PR1特异地受到SA诱导,而施加JA后抑制其表达;LOX2特异地受到JA的诱导,SA处理抑制其表达。上、下游基因的表达情况说明,PR1和LOX2可作为葡萄SA和JA信号转导途径的标记基因,同时研究发现葡萄中NPR1、PR1、COI1和LOX2表达量的快速上升出现在SA和JA处理后6~24h;SA与JA信号转导途径存在着协同或拮抗作用,它们之间的相对浓度决定着这种相互关系的变化。To understand gene response to exogenous signals in SA and JA signal transduction pathways of grapevine and analysis of the cross-interaction between these two pathways,this study cloned the genes of NPR1,PR1,COI1 and LOX2 from grapevine‘Fujiminori’（Vitis vinifera × Vitis labrusca）. The identity analysis of them with their homologous genes in different plants indicated that the related genes in SA and JA signaling pathways were conserved at relatively high level. Quantitative and semi-quantitative PCR were employed to analyze the expression of the four genes at different levels of hormone treatment,and the results showed that PR1 was specifically induced by the SA,and inhibited after JA applied;SA inhibited expression of LOX2（a gene specifically induced by JA）. According to expression of the two pairs of upstream and downstream genes belonging to SA and JA signalingpathways,the genes PR1 and LOX2 could be considered as the marker genes of these two signal transduction pathways in grapevine. The expression levels of NPR1,PR1,COI1 and LOX2 increased rapidly during the 6-24 h after treatment,and the antagonistic effect between SA and JA pathways may also exist in grapevine. The interactions between SA and JA pathways could be affected by the relative concentration of each hormone.

关 键 词：葡萄 水杨酸 茉莉酸 克隆 表达 

分 类 号：S663.1[农业科学—果树学]