RGD基序邻近氨基酸突变对口蹄疫病毒O/JC/2010株毒力的影响  

Role of the amino acids adjacent to RGD motif in the virulence of foot-and-mouth disease virus strain O/JC/2010

作　　者：况文东[1] 白兴文[1] 李平花[1] 祁国财[1] 包慧芳[1] 卢曾军[1] 孙普[1] 谢宝霞[1] 郝晓芳[1] 刘在新[1] 

机构地区：[1]中国农业科学院、兰州兽医研究所、家畜疫病病原生物学国家重点实验室、农业部畜禽病毒学重点实验室、国家口蹄疫参考实验室,甘肃兰州730046

出　　处：《中国兽医科学》2012年第5期441-447,共7页Chinese Veterinary Science

基　　金：公益性行业(农业)科研专项(201103008)

摘　　要：为了研究RGD基序邻近氨基酸位点对口蹄疫病毒毒力的影响,利用O/JC/2010株病毒的基因组全长cDNA分子克隆,置换O/HN/93株病毒的VP1基因,拯救获得了1株嵌合病毒(pBlue-HJ);除此之外,在O/JC/2010VP1的3个氨基酸位点进行突变P142T、A152D、Q153P,分为4组,拯救出4株突变病毒:pBlue-HJ(142)、pBlue-HJ(142+152)、pBlue-HJ(152+153)、pBlue-HJ(142+152+153)。比较这5株病毒的LD50和TCID50特性,发现P142T、A152D可以增强口蹄疫病毒对BHK-21细胞和乳鼠的致病力,而Q153P则相反。该试验证明RGD基序附近的非保守氨基酸位点对口蹄疫病毒的毒力存在影响,为进一步阐明口蹄疫病毒的分子致病机制奠定了基础。In order to study the role of neighboring amino acids of RGD motif in the virulence of footand-mouth disease virus（FMDV） ,the VPI coding region of O/JC/2010 strain was used to substituted for counterpart of genetically engineered virus O/HN/93. The chimeric FMDV strain（named pBlue-HJ） was got by reverse genetic technology. Besides, four chimeric FMDV strains pBlue-HJ （142）, pBlue-HJ （142 ＋ 152） ,pBlue-HJ（152＋ 153）, pBlue-HJ （142 ＋ 152 ＋ 153） were constructed through a serious of mutations （P142T,A152D and Q153P） in VP1. Mutations P142T and A152D were beneficial to virulence increasing in the BHK-21 cell and sucking mouse, nevertheless, Q153P went against virulence increasing. The results showed that the nonconservative amino acid sites adjacent to RGD motif could affect the virulence of FMDV. The study lays a foundation for further study of molecular pathogenesis of FMDV.

关 键 词：口蹄疫病毒 G-H环 RGD基序 毒力 

分 类 号：S852.659.6[农业科学—基础兽医学]