叶酸介导APTMS包被的超小超顺磁性氧化铁的制备及体外MR成像  

Preparation and in vitro MR imaging of folic acid-conjuncted APTMS coated ultrasmall superparamagnetic iron oxide

作　　者：潘迪[1] 严飞[2] 郑海荣[3] 吴瑞凤[1] 邱本胜[3] 

机构地区：[1]内蒙古工业大学化学工程学院化学工程与技术系,内蒙古呼和浩特010051 [2]中国科学院深圳先进技术研究院生物医学与健康工程研究所保罗.C.劳特伯生物医学成像研究中心 [3]中国科学院生物医学信息与健康工程学重点实验室,广东深圳518055

出　　处：《中国医学影像技术》2012年第5期838-842,共5页Chinese Journal of Medical Imaging Technology

摘　　要：目的探究叶酸介导的超小超顺磁性氧化铁(USPIO)对于人乳腺癌MCF-7细胞表面叶酸受体的靶向性及MR成像的可行性。方法①制备叶酸介导的耦联3-氨基丙基-三甲氧基硅烷(APTMS)的USPIO(FA-APTMS-US-PIO),通过TEM、FTIR等技术对其进行表征。②USPIO组、竞争抑制组(FA-APTMS-USPIO+叶酸)及叶酸介导的耦联APTMS的靶向组(FA-APTMS-USPIO)分别与MCF-7细胞孵育不同时间,通过普鲁士蓝染色观察不同组别铁颗粒的摄取情况。③对与纳米铁孵育后的细胞采用3.0TMR仪进行体外MR成像。④采用MTT法进行细胞活性检测。结果透射电镜照片显示FA-APTMS-USPIO外形较规则。普鲁士蓝染色观察,靶向组细胞摄取的氧化铁较多,可见大量蓝色沉淀。竞争抑制组与USPIO组细胞内蓝色颗粒较少。体外MRI结果显示,与MCF-7孵育的靶向组T2信号降低显著,竞争抑制组和USPIO组无明显变化。MTT实验显示,靶向组不同时间对细胞生存能力无显著影响。结论 FA-APT-MS-USPIO对MCF-7细胞有良好的靶向性,对早期诊断乳腺癌具有重大应用潜力。Objective To investigate the targeting efficiency of folic acid-conjunct APTMS-coated ultrasmall superparamagnetic iron oxide（FA-APTMS-USPIO） to MCF-7 cells via folic acid receptors,and to assess its potential application to MRI.Methods The following methods were used,including：①Preparation of FA-APTMS-USPIO and characterization with TEM and FTIR.②Plain USPIO group,FA-APTMS-USPIO group and competitive inhibition group（FA-APTMS-USPIO plus free folate acids） were setup in the experiment to be used to incubate with MCF-7 for 30 min,1 h and 2 h,respectively,followed by staining with Prussian blue solution and examined under microscope.③3.0T MR scanner was utilized to detect the signals of cells incubated with USPIO.④MTT assay was performed to examine the cell viability after USPIO incubation.Results TEM assay indicated the FA-APTMS-USPIO showed a uniform appearance.Much more blue iron oxide particles were observed in FA-APTMS-USPIO group,but just few were found in USPIO group or competitive inhibition group.In addition,MRI detection demonstrated significant decrease of T2 signal intensity for MCF-7 cells incubated with FA-APTMS-USPIO,while in control group and competitive group did not change obviously.MTT assay showed that the cytotoxicity did not obviously change in FA-APTMS-USPIO groups.Conclusion FA-APTMS-USPIO possesses a good targeting capability to MCF-7 cells and has great potential in early diagnose of breast tumor.

关 键 词：叶酸 纳米微粒 磁共振成像 

分 类 号：R445.2[医药卫生—影像医学与核医学]