单核细胞趋化蛋白-1诱导人主动脉平滑肌细胞表达基质金属蛋白酶-9及相关通路研究  

Effect of monocyte chemotactic protein-1 on matrix metalloproteinase-9 expression in human aortic smooth muscle cells and the related signaling pathways

作　　者：李晶[1] 张蓉[2] 赵力[2] 费宇行[2] 

机构地区：[1]北京第二军医大学海军临床医学院心脏中心,100048 [2]海军总医院心脏中心,北京100048

出　　处：《解放军医学杂志》2012年第5期464-466,共3页Medical Journal of Chinese People's Liberation Army

摘　　要：目的观察单核细胞趋化蛋白-1(MCP-1)对人主动脉平滑肌细胞(HASMCs)基质金属蛋白酶-9(MMP-9)蛋白表达的影响及其相关机制。方法将HASMCs细胞分为两部分,一部分分别加入0(对照组)、10、50、100ng/ml MCP-1干预48h,采用Western blotting法测定HASMCs细胞MMP-9、NF-κB p65的蛋白表达;另一部分先加入100ng/ml MCP-1,再分别加入0(对照组)、10、50、100μmol/L的NF-κB特异性抑制剂吡咯醛二硫氨基甲酸(PDTC),培养48h,采用Western blotting法检测HASMCs细胞MMP-9蛋白的表达情况。结果 MCP-1以剂量依赖方式刺激HASMCs细胞内MMP-9蛋白和NF-κB p65蛋白的表达,与对照组比较,加入10、50、100ng/ml MCP-1可使MMP-9蛋白的表达分别增加70.0%、118.3%和125.0%,使NF-κB p65蛋白的表达分别增加32.0%、52.0%和89.0%(P<0.01)。加入PDTC后,与对照组比较,10、50、100μmol/L的PDTC分别使MMP-9蛋白的表达降低了22.1%、28.9%和36.7%,均有显著性差异(P<0.01)。结论 MCP-1可诱导HASMCs表达MMP-9。NF-κB通路是MCP-1刺激MMP-9表达的调控通路之一。Objective To observe the effect of monocyte chemotactic protein-1 （MCP-1） on the expression of matrix metalloproteinase-9（MMP-9） in human aortic smooth muscle cells（HASMCs） and the related signaling pathways.Methods The HASMCs were divided into two parts.In the first part,0（control group）,10,50,and 100ng/ml MCP-1 were respectively added to interfere with MMP-9 expression for 48h;then,Western blot analysis was used to detect the protein expression of MMP-9 and NF-κB p65 in the HASMCs. In the second part, 100ng/ml MCP-1 was initially added, and then, 0 （control group）, 10, 50, and 100μmol/L of PDTC,as an NF-κB–specific inhibitor,was added to the cells.The cells were then cultured for 48h.Western blot analysis was used to detect MMP-9 expression in the HASMCs.Results The MMP-9 and NF-κB p65 expression in the HASMCs were dose-dependently stimulated by MCP-1. Compared with the control group, the addition of 10, 50, and 100ng/ml MCP-1 upregulated MMP-9 expression by 70.0%,118.3%,and 125.0%（P0.01）,and NF-κB p65 protein expression by 32.0%,52.0%,and 89.0%,respectively.The addition of 10,50,and 100μmol/L PDTC significantly downregulated MMP-9 expression by 22.1%,28.9%, and 36.7%,respectively（P0.01）.Conclusion MCP-1 induces MMP-9 expression in HASMCs.The NF-κB pathway regulates MCP-1 expression to stimulate MMP-9 expression.

关 键 词：趋化因子CCL2 基质金属蛋白酶9 NF-ΚB 

分 类 号：R543.12[医药卫生—心血管疾病]