卡立泊来德对K562细胞诱导的血管生成的影响  

Effect of cariporide on angiogenesis induced by K562 cells

作　　者：高伟 马丽 张洪菊 张玉娟 常国强 王建 李华文 庞天翔 

机构地区：[1]中国医学科学院、北京协和医学院、血液学研究所、血液病医院,实验血液学国家重点实验室,天津300020

出　　处：《中国药理学通报》2012年第5期617-620,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81170510);高等学校博士学科点专项科研基金资助项目(No 20091106110038)

摘　　要：目的研究卡立泊来德(cariporide)处理对K562细胞诱导的血管生成能力的影响。方法应用MTT检测K562细胞上清液对脐静脉内皮细胞增殖能力的影响;transwell检测K562细胞上清液对脐静脉内皮细胞迁移能力的影响;基质胶血管形成法检测K562细胞上清液对脐静脉内皮细胞体外血管形成能力的影响;激光扫描共聚焦显微镜测定K562细胞的细胞内的pH;酶联免疫吸附实验(ELISA)检测K562细胞上清中血管内皮生长因子的表达水平。结果cariporide处理可以明显降低K562细胞上清液对脐静脉内皮细胞增殖,迁移和体外成管能力的诱导;cariporide处理后K562细胞的细胞内pH明显下降,分泌VEGF能力也受到抑制。结论 cariporide能抑制K562细胞的血管生成诱导能力,这种抑制是通过细胞内pH下降以及VEGF分泌减少引起的。Aim To explore the effect of cariporide on angiogenesis induced by K562 cells.Methods MTT was used to measure the effect of K562 cell condition medium on human umbilical vein endothelial cells（HUVECs） proliferation;transwell was used to detect the effect of K562 cell condition medium on HUVECs migration;matrigel was used to explore HUVECs in vitro tube formation;intracellular pH（pH i） of K562 cells was analyzed by confocal laser scanning microscope;expression level of vascular endothelial growth factor（VEGF） in K562 cells condition medium was evaluated by enzyme linked immunosorbent assay.Results The proliferation,migration and in vitro tube formation of HUVEC were decreased dramatically in cariporide treated K562 cell condition medium;the pH i and VEGF expression of K562 cells were decreased upon cariporide treatment.Conclusions Cariporide can inhibit angiogenesis induced by K562 cells,and the inhibitory effect is induced by decreased pHi and reduced VEGF expression.

关 键 词：卡立泊来德 钠氢交换蛋白1 血管生成 细胞内pH 血管内皮生长因子 脐静脉内皮细胞 K562细胞 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R322.123[医药卫生—基础医学]