神经生长因子对精子活动力的影响  

Nerve growth factor improves sperm motility and vitality

作　　者：周晓东[1] 宋小骏[1] 袁力[1] 

机构地区：[1]南京军区南京总医院药品科,南京210002

出　　处：《医学研究生学报》2012年第4期341-343,共3页Journal of Medical Postgraduates

基　　金：国家自然科学基金(30770801)

摘　　要：目的影响精子活动力的因素较多。文中研究拟通过动物实验观察神经生长因子(never growth factor,NGF)对人精子活动力的影响,以找出更多的临床治疗弱精子症的手段并分析其机制。方法将NGF粉剂配成0、10、50、100μg/L 4个浓度溶液,作用于人精液30、60、90min后,观察精子活率和活动力的变化。大鼠肌注100μl(0.1μg)NGF,连续注射14 d,观察大鼠附睾尾精子数目和活力。结果 NGF处理精液60min后,浓度为50μg/L时活率显著高于正常对照组和100μg/L组(P<0.05),其他各组与对照组相比,精子活率差异均无统计学意义(P>0.05)。不同浓度NGF处理不同时间后,各组间精子活力差异无统计学意义(P>0.05)。NGF注射后,实验组大鼠附睾尾精子计数和精子活力明显增加,附睾尾精子相对计数显著高于对照组(P<0.05)。结论 NGF能促进精子的发生,对精子的活动力具有调控作用,有望为男性不育的治疗提供新思路。Objective Sperm motility can be affected by various factors.This study was to observe the effect of the nerve growth factor（NGF） on sperm motility in rats,analyze its mechanisms and find out more methods for the treatment of astenospermia.Methods Human sperm were exposed to the solution of NGF powder at the concentrations of 0,10,50 and 100 μg/L for 30,60 and 90 min,followed by detection of the changes in sperm motility and vitality.SD rats were intramuscularly injected with 100 μl NGF（0.1 μg） per day for 14 days,and then observed for the number and motility of sperm in the cauda epididymidis.Results After treated with NGF for 60 min,sperm motility was significantly higher in the 50 μg/L than in the 0 and 100 μg/L groups（P0.05）,but no statistically significant differences were observed in sperm vitality among different concentrations at different times（P0.05）.After NGF injection,both the number and motility of sperm in the cauda epididymidis were significantly increased in the experimental rats as compared with the controls（P0.05）.Conclusion NGF can improve spermatogenesis and sperm motility,and therefore provide some new ideas for the treatment of male sterility.

关 键 词：神经生长因子 精子活力 精子相对计数 

分 类 号：Q571[生物学—生物化学]