Application of mesenchymal stem cells as a vehicle to deliver replication-competent adenovirus for treating malignant glioma  

作　　者：Cui Hai Yong-Min Jin Wen-Biao Jin Zhe-Zhu Han Mei-Nv Cui Xue-Zhe Piao Xiong-Hu Shen Song-Nan Zhang Hong-Hua Sun 

机构地区：[1]Department of Oncology,Affiliated Hospital of Yanbian University,Yanji,Jilin 133000,P.R.China

出　　处：《Chinese Journal of Cancer》2012年第5期233-240,共8页

基　　金：supported by grants from the National Natural Science Foundation of China(No.81160275);the Claison tumor biotherapy research special funds(2011-14)

摘　　要：Although gene therapy was regarded as a promising approach for glioma treatment,its therapeutic efficacy was often disappointing because of the lack of efficient drug delivery systems.Mesenchymal stem cells(MSCs) have been reported to have a tropism for brain tumors and thus could be used as delivery vehicles for glioma therapy.Therefore,in this study,we attempted to treat glioma by using MSCs as a vehicle for delivering replication-competent adenovirus.We firstly compared the infectivity of type 3,type 5,and type 35 fiber-modified adenoviruses in MSCs.We also determined suitable adenovirus titer in vitro and then used this titer to analyze the ability of MSCs to deliver replication-competent adenovirus into glioma in vivo.Our results indicated that type 35 fiber-modified adenovirus showed higher infectivity than did naked type 3 or type 5 fiber-modified adenovirus.MSCs carrying replication-competent adenovirus significantly inhibited tumor growth in vivo compared with other control groups.In conclusion,MSCs are an effective vehicle that can successfully transport replication-competent adenovirus into glioma,making it a potential therapeutic strategy for treating malignant glioma.Although gene therapy was regarded as a promising approach for glioma treatment, its therapeutic efficacy was often disappointing because of the lack of efficient drug delivery systems. Mesenchymal stem cells （MSCs） have been reported to have a tropism for brain tumors and thus could be used as delivery vehicles for glioma therapy. Therefore, in this study, we attempted to treat glioma by using MSCs as a vehicle for delivering replication-competent adenovirus. We firstly compared the infectivity of type 3, type 5, and type 35 fiber-modified adenoviruses in MSCs. We also determined suitable adenovirus titer in vitro and then used this titer to analyze the ability of MSCs to deliver replication-competent adenovirus into glioma in vivo. Our results indicated that type 35 fiber-modified adenovirus showed higher infectivity than did naked type 3 or type 5 fiber-modified adenovirus. MSCs carrying replication-competent adenovirus significantly inhibited tumor growth in vivo compared with other control groups. In conclusion, MSCs are an effective vehicle that can successfully transport replication-competent adenovirus into glioma, making it a potential therapeutic strategy for treating malignant glioma.

关 键 词：脑胶质瘤 病毒复制 间质干细胞 腺病毒 治疗 能力 骨髓间充质干细胞 车辆 

分 类 号：Q939.4[生物学—微生物学] TH774[机械工程—仪器科学与技术]