D8／17抗原在风湿性心脏病患者中的表达及特性  

The B lymphocytes D8/17 antigen expression and characteristics of patients with rheumatic heart disease in Guangdong

作　　者：陈剑光[1] 董太明[2] 陈志红[1] 李荣[1] 苏健[1] 吴红穗[1] 

机构地区：[1]广东省人民医院医学研究中心广东省医学科学院,广州510080 [2]广东省人民医院心内科,广州510080

出　　处：《中华内科杂志》2012年第5期353-356,共4页Chinese Journal of Internal Medicine

基　　金：广东省医学科研基金课题（A2007007）;广东省科技计划项目（2004B30601008）.志谢 美国洛克菲勒大学Zabriskie JB教授热情提供D8/17单克隆抗体并指导实验;广东省人民医院检验科流式细胞仪室张建军、周茂华主任及全室人员支持和帮助

摘　　要：目的 探讨D8/17抗原在风湿性心脏病（RHD）患者的表达情况，并对该抗原的特性进行初步研究。 方法 应用白种人制备的D8/17单克隆抗体，通过流式细胞术分析96例RHD患者和83例对照组患者B淋巴细胞中D8/17抗原的表达率，以表达率≥10%判为D8/17阳性。用免疫共沉淀法提取D8/17抗原，用串联质谱技术分析D8/17抗原的特性。 结果 D8/17抗原在RHD患者和对照组患者B淋巴细胞中的表达率分别为（85.36±15.15）%和（82.89±14.55）%，两组间差异无统计学意义（P=0.436），且两组人群D8/17阳性率均为100%。 D8/17抗原的相对分子质量约为40 000-67 000，最具可能匹配的蛋白是膜突蛋白和β-肌动蛋白。结论 本研究未提示D8/17抗原与RHD相关；D8/17抗原最具可能匹配的蛋白是膜突蛋白或β-肌动蛋白。Objective To investigate the D8/17 antigen expression of patients with rheumatic heart disease （RHD） in Guangdong province and study the antigen′s characteristics.Methods The level of D8/17 antigen expression on B lymphocytes was determined with flow cytometry assay in 96 RHD patients and 83 unaffected controls. The percentage of B-cells expressing the D8/17 antigen having more than 10% was considered to be positive. D8/17 antigen was extracted by immunoprecipitation, and the antigen characteristics was analyzed by tandem mass spectrometry. Results The mean percentage of B-cells expressing the D8/17 antigen was （85.36±15.15）% in the RHD patients and （82.89±4.55）% in the controls, with no significant difference between the two groups（P=0.436）. Moreover, the positive rate of the D8/17 expression was 100% in either the RHD patients or the controls. The molecular weight of D8/17 antigen was found to be 40 000-67 000, and the purified protein was most likely to match moesin or β-actin. Conclusions B-cell antigen D8/17 is not associated with RHD in Guangdong province of China. Moesin or β-actin is the most likely protein to match D8/17 antigen.

关 键 词：风湿性心脏病 生物学标志 D8/17抗原 

分 类 号：R541.2[医药卫生—心血管疾病]