高增殖潜能内皮祖细胞促进脐血造血祖细胞体外扩增  

High proliferative potential endothelial progenitor cells support the expansion of hematopoietic progenitor cells from human umbilical cord blood

作　　者：段华新[1,2] 刘畅[1,2] 杨新宇[1,2] 毛平[3] 徐艳丽[3] 罗畅如[3] 王彩霞[3] 谢健晋[3] 张玉平[3] 

机构地区：[1]湖南师范大学第一附属医院 [2]湖南省人民医院血液肿瘤科,湖南长沙410006 [3]广州市第一人民医院血液科,广东广州510180

出　　处：《广州医学院学报》2012年第1期1-4,共4页Academic Journal of Guangzhou Medical College

基　　金：中国博士后科学基金(20070420762);湖南省自然科学基金(07JJ5034);长沙市科技计划(1-10308);湖南省教育厅科学研究基金(09C633);湖南省人民医院仁术基金

摘　　要：目的:研究脐血来源高增殖潜能内皮祖细胞支持脐血造血干/祖细胞体外扩增的能力。方法:利用第3代的HPP-EPC联合细胞因子培养脐血造血干/祖细胞,分因子组、非接触培养组和接触培养组。体外培养脐血CD34^+细胞10 d后,从细胞总数,CD34^+细胞数,各系造血集落形成细胞数等比较各组的扩增效率。结果:接触组细胞总数扩增倍数(44.6±8.7)倍和非接触组(39.5±7.3)倍均高于因子组(24.8±5.6)倍,P<0.01。接触组CD34^+细胞数扩增倍数(8.8±2.1)倍高于因子组(2.9±0.6)倍和非接触培养组(3.3±0.6)倍,P<0.01。接触组集落扩增倍数和非接触组均分别高于因子组,P<0.05,且接触组各造血集落扩增数均分别高于非接触组,各P<0.05。接触组扩增后CD34^+CD38^-细胞(10.8%±2.7%)明显高于因子组(4.1%±0.9%)和非接触培养组(3.8%±0.8%),P<0.01。结论:脐血来源高增殖潜能内皮祖细胞能支持脐血CD34^+细胞体外扩增。Objective： To investigate whether human cord blood derived high proliferative potential endothelial progenitor cells （HPP-EPC） can support the expansion of heraatopoietie progenitor cells from human umbilical cord blood. Methods： Passage 3 HPP-EPCs were used to expand CD34 ＋ cells from fresh umbilical cord blood in 3 groups ： cytokines group as control, non contact group and contact group. After 10 d culture, total cell number, CD34 ＋ cell number, colonies of hematopoietic progenitors were counted to analyze the expansion effects in each group. Results： After 10 d expansion, the expansion folds of total eell number in both contact group （44.6 ± 8.7 ） and non contact group （39.5 ± 7.3 ） were higher than in cytokine group （24.8 ± 5.6, both P 〈 0.01 ）. The expansion fold of CD34 ＋ was higher in contact group （ 8.8 ± 2.1 ） than in non contact group （ 3.3 ± 0.6 ） or cytokine group （ 2.9 ± 0.6 ） （ both P 〈 0.01 ）. The expansion folds of colonies in contact group and in non contact group were higher than those in cytokine group （ all P 〈 0.05 ）. And the expansion folds of colony number of hematopoietic progenitors in contact group were higher than those in non contact group （ all P 〈0.05）. The rate of CD34 ＋ CD38- cells after expansion ex vivo in contact group （10.8% ± 2.7% ） was higher than that in cytokine group （4.1%± 0.9% ） and non contact group （3.8% ± 0.8% ）. Conclusion： Human umbilical cord blood derived HPP-EPC can support the expansion of CD34 ＋ hematopoietic stem/progenitor cells from human umbilical cord blood.

关 键 词：高增殖潜能内皮祖细胞 脐血 造血干/祖细胞 扩增 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学]