纳米银局部给药治疗脊柱化脓性感染的实验研究  

Experimental study of local administration of silver nanoparticles for the treatment of pyogenic spondylitis

作　　者：李晶[1] 唐钦[1] 邓露[2] 王孝宾[1] 盖景颖[3] 王明[1] 杨明[1] 

机构地区：[1]中南大学湘雅二医院脊柱外科,长沙410011 [2]中南大学湘雅二医院手术室,长沙410011 [3]湖南省第二人民医院脊柱外科,长沙410007

出　　处：《中南大学学报（医学版）》2012年第4期374-378,共5页Journal of Central South University ：Medical Science

基　　金：湖南省自然科学基金(09JJ6033)~~

摘　　要：目的:建立合适的脊柱化脓性感染动物模型,探讨纳米银局部给药治疗脊柱化脓性感染的疗效,以及局部给药后纳米银在动物体内的生物学分布。方法:将金黄色葡萄球菌经椎间隙接种建立兔的脊柱化脓性感染模型。建模成功后连续2周每日局部给予不同剂量的纳米银水溶液,分实验组A[0.1 mg/(kg.d)],实验组B[1 mg/(kg.d)]和生理盐水对照组,观察感染部位的影像学和组织学改变。再应用原子吸收光谱法测量实验动物重要脏器的银含量,分析纳米银在动物体内的分布情况。结果:金黄色葡萄球菌(100 CFU/mL)能100%诱导出脊柱的化脓性感染。1 mg/kg剂量纳米银能有效地抑制脊柱化脓性感染的发生,有效率为75%;而0.1 mg/kg剂量纳米银抑制脊柱化脓性感染发生的效果不明显,有效率仅为25%。高浓度(1 mg/kg)纳米银会在脊髓、肝、肾、脾、睾丸产生蓄积,低浓度(0.1 mg/kg)纳米银仅在脊髓产生蓄积。结论:纳米银局部连续给药能有效预防和治疗脊柱的化脓性感染,但在动物体内会产生蓄积作用,并且与给药浓度相关。Objective：To establish a proper animal model of pyogenic spondylitis,to evaluate the efficacy of silver nanoparticles for treatment of spinal pyogenic infections,and to explore the distribution of the particles in the body of the animals.Methods：Staphylococcus aureus was inoculated into intervertebral discs of rabbits to establish a spinal pyogenic infection model.These rabbits were divided into Group A [0.1 mg/（kg·d）],Group B [1 mg/（kg·d）],and a Control group.Groups A and B were injected with different doses of silver nanoparticles solution daily at locally infected side.Two weeks later,bacterial cultures,radiographic outcomes,histopathology were analyzed.Atomic absorption spectrometry was utilized to measure silver contents in some vital organs of the rabbits to detect the distribution and accumulation of silver particles.Results：Staphylococcus aureus（100 CFU/mL）,induced 100% pyogenic spondylitis.1 mg/kg dose of silver nanoparticles solution could effectively inhibit the occurrence of spinal pyogenic infection with the effective rate of 75%.While the effect of 0.1 mg/kg dose of silver nanoparticles solution was less obvious,the efficiency was only 25%.Significant numbers of silver nanoparticles were observed to accumulate in the animal.In the 1 mg/kg group silver was deposited mainly in the spinal cord,liver,kidneys,spleen,and testicles,while in the 0.1 mg/kg group it accumulated only in the spinal cord.Conclusion：Local administration of silver nanoparticles can effectively prevent and treat pyogenic spondylitis;the particles accumulate in the body commensurate with the administered drug concentration.

关 键 词：纳米银 化脓性脊柱炎 抗菌剂 生物安全性 

分 类 号：R681.5[医药卫生—骨科学]