对扫描电镜观察微生物样品出现磷酸盐结晶现象的改良  

The improvement for the phenomena of phosphate crystallization during making microbiological samples for scanning electron microscope observation

作　　者：梁静南[1] 刘一苇[1] 

机构地区：[1]中国科学院微生物研究所,北京100101

出　　处：《电子显微学报》2012年第2期194-196,共3页Journal of Chinese Electron Microscopy Society

摘　　要：制备常规扫描电镜生物样品时,通常使用磷酸缓冲液来进行漂洗,其结果是容易产生结晶,沉积在样品表面。针对微生物具有坚韧的细胞壁的特性,作者使用去离子水替代磷酸缓冲液来漂洗固定液。结果显示,扫描电镜下样品形态完好,无磷酸盐结晶,提高了样品制备的成功率。During the step of rinsing biological samples for SEM observation,the PBS buffer is usually used and it is easily crystallized on the surface of samples so as to cover the structure.For microbiology with the characteristics of the cell wall,We use the deionized water instead of the PBS buffer to rinse the fixative so that not only the sample shape is without distortion but also the surface of the sample is clean which is better for observation.

关 键 词：微生物 磷酸盐结晶 扫描 

分 类 号：Q93[生物学—微生物学]