类风湿关节炎患者外周血TCRαβ^+CD4^-CD8^-T细胞与FAS介导的凋亡关系研究  

Relationship between peripheral TCRαβ^+CD4^-CD8^-T cells and FAS-mediated apoptosis in patients with rheumatoid arthritis

作　　者：王红霞[1] 裘宇容[1] 包杰[1] 李娟[2] 赖伟男[2] 

机构地区：[1]南方医科大学南方医院临床检验中心,广州510515 [2]南方医科大学南方医院风湿免疫科,广州510515

出　　处：《重庆医科大学学报》2012年第4期311-314,共4页Journal of Chongqing Medical University

基　　金：2011广东省自然科学基金资助项目(编号:S2011010005368)

摘　　要：目的:检测类风湿关节炎(Rheumatoid arthritis,RA)患者外周血中TCRαβ+细胞、CD4-CD8-细胞、TCRαβ+CD4-CD8-T细胞(Double negative T cells,DNT细胞)及各细胞亚群表面凋亡相关蛋白FAS的表达情况,探讨RA患者TCRαβ+CD4-CD8-T细胞与FAS介导的凋亡之间的关系。方法:选取24例RA患者以及24例同期健康查体者作为研究对象,检测所有研究对象外周血TCRαβ+细胞、CD4-CD8-细胞、TCRαβ+CD4-CD8-T细胞的数量及表面凋亡相关蛋白FAS的表达情况,并分析以上指标与类风湿因子(Rheumatoid factors,RF)、白细胞数(White blood cell,WBC)、急性时相反应蛋白(C-reactive pro-tein,CRP)以及血沉(Erythrocyte sedimentation rate,ESR)之间的相关性。结果:RA患者外周血TCRαβ+细胞、CD4-CD8-细胞与健康对照相似,TCRαβ+CD4-CD8-T细胞数量显著高于健康对照(0.82±0.38 vs.0.57±0.26,P=0.013),TCRαβ+CD4-CD8-T细胞表面的FAS的表达增高(1.23±0.69 vs.0.80±0.45,P=0.016),并与TCRαβ+CD4-CD8-T细胞数量呈显著正相关关系(r=0.809,P=0.000);TCRαβ+细胞及其表面表达的FAS受体、CD4-CD8-细胞及其表面表达的FAS受体、TCRαβ+CD4-CD8-T细胞及其表面表达的FAS受体与RF、WBC、CRP以及ESR之间均无相性(P>0.05)。结论:RA患者外周血中TCRαβ+CD4-CD8-T细胞及其表面表达的FAS受体显著增加,表明TCRαβ+CD4-CD8-T细胞对FAS介导的凋亡敏感性增加,可能会导致免疫反应性T细胞的比例失调。RA患者中TCRαβ+CD4-CD8-T细胞及其表面表达的FAS受体与疾病活动性指标之间无相关关系,TCRαβ+CD4-CD8-T细胞及其表面表达的FAS受体在RA患者的发病机制中的作用还有待于进一步探讨。Objective：To investigate the expression of FAS receptor on idiosyncratic T cell subsets,including TCRαβ＋cells,CD4-CD8-cells and TCRαβ＋CD4-CD8-T cells,and to discuss the relationship between FAS-mediated apoptosis and peripheral TCRαβ＋CD4-CD8-T cells in patients with rheumatoid arthritis（RA）.Methods：Totally 24 RA patients and 24 normal controls were included.Flow cytometry was used to detect the percentages of T cell subsets and the expression of FAS receptor.The relationships between the percentages of T cell subsets and the expression of FAS receptor with RF,WBC,CRP and ESR were analyzed respectively by spearman relativity analysis.Results： The percentages of peripheral TCRαβ＋ cells and CD4-CD8-cells from RA patients were similar to those in normal controls（P0.05）.Strikingly higher levels of TCRαβ＋CD4-CD8-T cells in the peripheral blood of RA patients were detected compared with those in normal controls（0.82±0.38 vs.0.57±0.26,P=0.013）.The expressions of FAS receptor on TCRαβ＋CD4-CD8-T cells were significantly increased in RA patients compared with those in normal controls（1.23±0.69 vs.0.80±0.45,P=0.016）.Positively relationship was found between TCRαβ＋CD4-CD8-T cells and TCRαβ＋CD4-CD8-FAS＋cells（r=0.809,P=0.000）.There was no significant relationship between the percentages of TCRαβ＋CD4-CD8-T cells,the level of FAS receptor expression and RF,WBC,CRP or ESR,respectively.TCRαβ＋ cells as well as CD4-CD8-cells had no significant association with RF,WBC,CRP and ESR,respectively.Conclusion： The preliminary results suggest that the significantly upregulated percentages of DNT cells and its FAS receptor expression may increase the sensitivity to FAS-mediated apoptosis.It may lead to the imbalance of immune respective T cells in RA patients.There is no significant relationship between the percentages of TCRαβ＋CD4-CD8-T cells,FAS receptor and the indicators of disease activity,though the percentages of them are markedly increased in the Chinese RA

关 键 词：类风湿性关节炎 TCRαβ+CD4-CD8-T细胞 FAS 细胞凋亡 

分 类 号：R593.22[医药卫生—内科学]