骨关节炎时内源性细胞因子及基质金属蛋白酶抑制因子1对关节软骨的影响  

Effect of endogenous cytokines and tissue inhibitor of matrix metalloproteinase 1 on the articular cartilage of osteoarthritis patients

作　　者：赵敬河[1] 杨秋云[1] 王冰[2] 

机构地区：[1]哈尔滨医科大学附属第五医院,黑龙江省大庆市163316 [2]哈尔滨医科大学大庆校区,黑龙江省大庆市163316

出　　处：《中国组织工程研究》2012年第15期2665-2668,共4页Chinese Journal of Tissue Engineering Research

基　　金：黑龙江省卫生厅资助项目(2004-043)~~

摘　　要：背景:研究发现,细胞因子通过影响关节软骨基质的分解代谢和合成代谢的平衡来参与骨关节炎的形成,其中白细胞介素1β、基质金属蛋白酶抑制因子1在其中起重要作用。目的:观察内源性细胞因子白细胞介素1β及基质金属蛋白酶抑制因子1与骨关节炎关节软骨退变的关系。方法:纳入2006-06/2009-09于哈尔滨医科大学附属第五医院就诊的原发性骨关节炎患者37例,在患者行关节镜检时抽取关节液及分离平滑光亮的滑膜组织。另取10例正常关节液标本及平滑光亮的滑膜组织标本作为对照。结果与结论:ELISA法检测结果显示骨关节炎患者关节液和滑膜组织中白细胞介素1β、基质金属蛋白酶抑制因子1水平均显著高于正常水平,且患者骨关节炎越严重,关节液和滑膜组织中白细胞介素1β、基质金属蛋白酶抑制因子1的水平越高。说明关节液中白细胞介素1β、基质金属蛋白酶抑制因子1水平与骨关节炎、关节软骨退变有关。BACKGROUND：Studies have found that the cytokine participates in the formation of osteoarthritis by influencing balance between catabolism and anabolism of articular cartilage matrix.Interleukin 1β（IL-1β） and tissue inhibitors of matrix metalloproteinase 1（TIMP-1） play an important role.OBJECTIVE：To investigate the effect of endogenous cytokines,IL-1β and TIMP-1,on cataplasia of articular cartilage of osteoarthritis.METHODS：Thirty-seven patients with knee osteoarthritis were studied who were treated in the Fifth Affiliated Hospital of Harbin Medical University during 2006-06/2009-09.Extraction of synovial fluid and separation of smooth and shining synovial membrane tissues were performed during microscopic examination in patients.Ten specimens of normal synovial fluid and smooth and shining synovial membrane tissue were used as control.RESULTS AND CONCLUSION：In the osteoarthritis patients,the levels of IL-1β and TIMP-1 in synovial fluid and synovial membrane tissue were all significantly higher than those in the normal synovial fluid,the synovial fluid concentration of IL-1β and TIMP-1 were positively related to the severity of osteoarthritis.These findings demonstrate that the level of IL-1β and TIMP-1 are related with osteoarthritis and cartilage degradation.

关 键 词：基质金属蛋白酶抑制因子1 骨关节炎 白细胞介素1Β 关节液 滑膜组织 关节软骨退变 

分 类 号：R318[医药卫生—生物医学工程]