跟腱损伤模型大鼠接受低频脉冲超声与激素封闭治疗的比较  

Low-frequency pulsed ultrasound versus hormone blockade for the treatment of tendinopathy in rats

作　　者：徐西东[1] 刘伟[1] 刘淼[1] 斯海波[1] 陈世荣[1] 

机构地区：[1]重庆医科大学附属第二医院骨科,重庆市400010

出　　处：《中国组织工程研究》2012年第15期2762-2766,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:低频脉冲超声已应用于多种慢性软组织损伤的治疗。目的:制造大鼠跟腱病模型,观察低频脉冲超声治疗效果,并与激素封闭相比较。方法:60只SD大鼠随机抽签分为空白对照组(n=10)及造模组(n=50),后者用电击跳跃法造模8周制备跟腱病模型,造模成功后分别进行低频超声、局部封闭或单纯休息处理4周,记录其跳跃次数,行病理切片苏木精-伊红染色、天狼星染色及Tunel法细胞凋亡检测。结果与结论:造模过程中大鼠跳跃能力先增强后下降,造模成功后大鼠跟腱潮线上涨、Ⅰ/Ⅲ型胶原比例倒置、肌腱细胞凋亡增多;低频超声治疗组大鼠跟腱胶原比例明显恢复,凋亡细胞减少,而激素封闭组和休息组未见明显改善;说明低频脉冲超声可用于治疗跟腱病,对跟腱具有保护作用。BACKGROUND：Low-frequency pulsed ultrasound is widely used in the treatment of chronic soft tissue injuries.OBJECTIVE：To establish a rat model of tendinopathy and to observe the therapeutic effects of low-frequency pulsed ultrasound versus hormone blockade.METHODS：Totally 60 Sprague-Dawley rats were randomly divided into two groups：black control group（10 rats） and experimental group（50 rats） which received electric stimulus for 8 weeks to construct the rat models of tendinopathy.After modeling successfully,low frequency ultrasound,corticosteroid local blocking,and 4 week simple rest were performed.The number and height of jumping were recorded.Hematoxylin-eosin staining,sirius-red staining and Tunnel staining were used to observe the pathological changes.RESULTS AND CONCLUSION：Jumping ability of the rats firstly increased then decreased during modeling.After modeling successfully,Achilles tendon tide line increased,Ⅰ/Ⅲ type collagen ratio inversed and apoptosis increased.In the low frequency ultrasound group,the Achilles tendon collagen ratio was obviously recovered and apoptosis was decreased,but which in the hormone blockade and simple rest groups had no obvious changes.It is suggested that low-frequency pulsed ultrasound can be used for the tendinopathy treatment,and has a protective effect on Achilles tendon.

关 键 词：低频脉冲超声 肌腱病 电击跳跃 大鼠跟腱 胶原纤维 凋亡细胞 

分 类 号：R318[医药卫生—生物医学工程]