新型纳米化仿生骨基质与羊脂肪基质细胞复合培养的生物相容性  

Biocompatibility of new nano-structured biomimetic bone matrix co-cultured with goat adipose derived stromal cells

作　　者：郭洪刚[1] 刘静[2] 李峰坦[1] 杨少光 陈治[4] 姚芳莲[5] 代凤英[6] 刘文广[6] 

机构地区：[1]天津医科大学总医院骨科,天津市300052 [2]天津市中心妇产科医院,天津市300052 [3]中国医学科学研究院血液病研究所,天津市300052 [4]天津大学精密学院,天津市300072 [5]天津大学化工学院,天津市300072 [6]天津大学材料学院,天津市300072

出　　处：《中国组织工程研究》2012年第16期2889-2892,共4页Chinese Journal of Tissue Engineering Research

基　　金：国家自然基金资助项目(81041061);天津市教委科技基金资助项目(20090125)~~

摘　　要：背景:仿生策略研制骨组织生物支架已成为当今研发生物替代品的关键环节,如何模仿天然骨的成分和结构特征以适应细胞生长是仿生研制骨基质的重要内容。目的:观察纳米级β-磷酸三钙/壳聚糖/聚己内酯(nβ-TCP-CS-PCL)的细胞及组织相容性,评估其生物安全性。方法:基于三维图像重建辅助研制纳米化仿生骨基质,羊自体脂肪基质细胞分离和成骨诱导培养,进行复合培养:实验组:nβ-TCP-CS-PCL+凝胶+重组人骨形态发生蛋白2+脂肪基质细胞;对照组:凝胶+重组人骨形态发生蛋白2+脂肪基质细胞。结果与结论:实验组细胞生长旺盛、细胞外基质丰富,细胞增殖活力、碱性磷酸酶及骨钙素水平均高于对照组(P<0.05),新骨发育趋于成熟,未见炎性反应。提示纳米级nβ-TCP-CS-PCL具备良好的生物相容性,是一种理想的骨组织工程支架。BACKGROUND： Development of a bone tissue scaffold using biomimetic strategy has become the key link in development of biological substitutes currently. Imitation of natural bone composition and structure is important in development of bone matrix using biomimetic strategy. OBJECTIVE： To analyze the biocompatibility of nano-scaled β-tricalcium phosphate/chitosan/polycaprolactone （nβ-TCP-CS-PCL） and to evaluate its biological safety. METHODS： Nanostructured bionic bone matrix was developed based on three-dimensional image reconstruction. Goat adipose derived stromal cells were isolated and co-cultured for osteogenic induction. The experimental group： nβ-TCP-CS-PCL＋gel＋ recombinant human bone morphogenetic protein-2＋adipose derived stromal cells. The control group： gel＋recombinant human bone morphogenetic protein-2＋adipose derived stromal cells. RESULTS AND CONCLUSION： Cells grew well with abundant extracellular matrix in the experimental group. The cell proliferation activity, alkaline phosphatase level and osteocalcin level were increased in the experimental group compared with the control group （P〈0.05）. New bone was gradually mature and there was no inflammatory response in the experimental group. The nβ-TCP-CS-PCL can be used as an ideal scaffold of bone tissue engineering for its good biocompatibility.

关 键 词：仿生骨基质 脂肪基质细胞 生物相容性 β-磷酸三钙/壳聚糖/聚己内酯 纳米级 

分 类 号：R318[医药卫生—生物医学工程]