氟纳米羟基磷灰石封闭人离体牙牙本质小管的生物相容性  

Biocompatibility of dentinal tubules in human extracted teeth closed with fluoride nano-hydroxyapatite

作　　者：王健平[1] 周立波[2] 岳红霞[3] 

机构地区：[1]佳木斯大学附属第二医院,黑龙江省佳木斯市154007 [2]佳木斯大学口腔医学院,黑龙江省佳木斯市154002 [3]佳木斯大学附属第二医院牙体二科,黑龙江省佳木斯市154007

出　　处：《中国组织工程研究》2012年第16期2951-2954,共4页Chinese Journal of Tissue Engineering Research

摘　　要：背景:氟纳米羟基磷灰石具有抗酸性,与牙体组织成分相似,生物相容性好,对牙釉质具有再矿化功能等特点。目的:观察氟纳米羟基磷灰石对牙本质小管的封闭效果。方法:选取56颗健康离体牙,随机分为4组,分别为纳米含氟羟基磷灰石组、纳米羟基磷灰石组、氟化钠组及空白对照组。实验各组每天早晚分别用3组材料涂刷牙本质小管外层的牙本质2次,2min/次,置于人工唾液37℃恒温箱中保存;7d后将牙纵向劈开为即刻组,模仿人日常刷牙方法用去离子水涂刷实验区100次再将其纵向劈开为磨损组,扫描电镜观察牙本质表面实验区和剖面牙本质小管堵塞率及封闭深度。结果与结论:空白组牙本质小管完全开放,扫描电镜观察氟纳米羟基磷灰石对牙本质小管的封闭效果明显优于其他3组;即刻组和磨损组封闭效果均为纳米含氟羟基磷灰石>纳米羟基磷灰石>氟化钠。结果显示纳米含氟羟基磷灰石小管堵塞效果和封闭深度均显著高于纳米羟基磷灰石﹑氟化钠材料。BACKGROUND： Fluoride nano-hydroxyapatite has acid resistance, which is similar to the composition of tooth tissues. The fluoride nano-hydroxyapatite has good biocompatibility and has functional characteristics of remineralization on enamel. OBJECTIVE： To observe the closed effect of fluoride nano-hydroxyapatite on dentinal tubules. METHODS： Fifty-six healthy extracted teeth were randomly divided into four groups： fluoride nano-hydroxyapatite, nano-hydroxyapatite, sodium fluoride and blank control groups. Materials in each group were used to coat outer dentinal tubules twice on every morning and evening respectively （2 minutes/time）, and then were saved in artificial saliva in a 37 ℃ thermostat. The teeth were longitudinal split as immediate group at 7 days after experiment. Teeth were longitudinal split again as wear group which using deionized water to simulate daily brushing 100 times on experimental area. Experimental area of dentin surface, blocking rate and closed depth of the section of dentinal tubules were observed using scanning electron microscope. RESULTS AND CONCLUSION： The dentinal tubules were open fully in the blank control group. Scanning electron microscope results showed that sealing effect of the dentinal tubules in the fluoride nano-hydroxyapatite group was better than that in the other three groups. Sealing effect of the dentinal tubules in the immediate and wear groups were fluoride nano-hydroxyapatite 〉 nano-hydroxyapatite 〉 sodium fluoride. It is indicated that blocking effect and sealing depth of the fluoride nano-hydroxyapatite are higher than those of the nano-hydroxyapatite and sodium fluoride.

关 键 词：氟纳米羟基磷灰石 纳米羟基磷灰石 牙本质敏感 氟化钠 扫描电镜 

分 类 号：R318[医药卫生—生物医学工程]