三金汤防治化学纯三聚氰胺尿结石及对肾脏保护作用的研究  

Effects of San Jin Tang on the treatment of melamine associated urinary stones

作　　者：张薇[1] 邹移海[2] 陈嘉[2] 张钟广[2] 黎福荣[1] 

机构地区：[1]中山大学实验动物中心,广东广州510080 [2]广州中医药大学实验动物中心,广东广州510405

出　　处：《热带医学杂志》2012年第4期436-439,477,F0002,共6页Journal of Tropical Medicine

基　　金：国家重点基础研究发展计划973计划课题(2005CB523502);广东省科技基础条件建设项目(2009B060300003);广州市科技计划项目(2009J1-C461)

摘　　要：目的观察三金汤防治SD大鼠三聚氰胺尿结石及对肾脏的保护作用。方法 120只SD雄性大鼠随机分为8组,即预防组(高、中、低剂量组)、治疗组、空白对照1、2组、模型对照1、2组。0.4g/kg化学纯三聚氰胺给药20d诱导SD大鼠尿结石,三金汤预防高、中、低剂量组28.4g/kg、14.2g/kg、7.1g/kg连续灌胃三金汤35d;治疗组在造模20d后给予28.4g/kg三金汤15d,模型2组在造模20d后灌胃无菌水1ml/只/d,共15d,空白对照1、2组灌胃无菌水1ml/只/d,分别20d和35d,采用体视显微镜观察大鼠肾脏、输尿管和膀胱结石及形态改变,比较各组肾脏、膀胱的质量和脏器指数,观察肾功能指标血清肌酐CREA、尿素氮BUN含量的变化。结果预防高、中剂组结石最少,均为1例,预防低剂组2例,治疗组3例,模型2组5例,模型1组6例,空白对照1、2组未见结石形成;血清CREA、BUN预防组、治疗组与空白对照1、2组比较差异无统计学意义;但血清CREA治疗组[(35.10±2.81)μmol/L]比预防高剂量组[(28.40±3.27)mmol/L]高,差异有统计学意义(P<0.05);模型2组血清CREA[(38.3±10.14)μmol/L]比空白对照1、2组[(31.20±3.26)、(31.5±4.30)μmol/L]、预防中剂量组[(32.20±5.88)μmol/L]高(P<0.05),比模型1组[(29.10±5.76)μmol/L]、预防高、低剂量组[(28.40±3.27)、(29.80±7.55)μmol/L]高(P<0.01);模型2组血清BUN[(6.26±0.78)mmol/L]比空白对照1、2组[(5.06±1.21)、(5.46±0.75)mmol/L]、预防组[(5.40±076)、(5.08±1.31)、(5.17±0.65)mmol/L]高(P<0.05)。结论三金汤具有预防、治疗三聚氰胺尿结石的作用;三聚氰胺沉淀自行排出缓慢,停止造模后其对肾组织所引起的炎性刺激尚未消除,对大鼠肾功能有蓄积性、迟发性损害;同时三金汤能有效预防三聚氰胺在肾组织中的积聚、沉淀以及对肾功能的损害,预防作用比治疗作用效果好。Objective To investigating the effect of the Chinese Medicine ＂San Jing Tang＂ on the prevention and cure of urinary calculus induced by melamine in SD rats. Methods 80 male SD rats were randomly divided into 8 groups, namely the preventative group （high, middle, low dosage group）, treatment group, blank control group 1 and group 2, and model control group 1 and group 2. A dosage of 0.4 g/kg melamine was administrated to rats for 20 days to induce the formation of uric calculus..Various doses of San Jing Tang [（28.4 g/kg （high group）, 14.2 g/kg （middle group） and 7.1 g/kg（low group）], were intragastrally administrated for 35 days to the rats in the preventive groups. Rats in the treatment group received 28.4 g/kg San Jing Tang for 15 day after the administration of melamine..Rats in the model control group were intragastrally administrated with water（1 ml/d）for 15 days after the administration of melamine.Rats in the blank group were administrated with water for 35 days.The shape of the calculus in kidney,ureter and bladder was examined under a stereo microscope,and the weight of the kidney and bladder was recorded to evaluate the size of the calculus. Renal function was determined by measuring the levels of blood creatinine （CREA） and urea nitrogen （BUN）. Results Both the high dosage preventative group and middle dosage preventative group had 1 rat with urinary calculus. Two rats in the low dosage preventive group had urinary calculus..The number of rats with urinary calculus in the treatment group, model control group 2 and model control group 1 was 3, 5 and 6, respectively.Our data didn＇t show any difference about the level of CREA and BUN when comparing the preventive groups and treatment group with the blank control group, while the level of CREA in the treatment group [（35.10±2.81） μmol/L] was higher than the high dosage preventive group [（28.40±3.27） μmol/L,P0.05]. The level of CREA in the model group-2 [（38.3±10.14）μmol/L] was higher than the two blank

关 键 词：三聚氰胺 三金汤 尿结石 SD大鼠 

分 类 号：R694.4[医药卫生—泌尿科学]