过氧化物酶增殖激活受体γC161T基因多态性与低出生体质量新生儿的关系  

Correlation between Peroxisome Proliferator-Activated Receptor γ C161T Gene Polymorphism and Low Birth Weight Infants

作　　者：刘文静[1] 陈守航[1] 高莉丽[1] 张培林[2] 张华[2] 陈英[2] 郑明慈[1] 

机构地区：[1]桂林医学院附属医院围产医学研究室,广西桂林541001 [2]桂林医学院附属医院新生儿科,广西桂林541001

出　　处：《实用儿科临床杂志》2012年第8期591-593,共3页Journal of Applied Clinical Pediatrics

基　　金：中国疾病预防与控制中心达能营养中心膳食营养研究与宣教基金(DIC2009-12)

摘　　要：目的探讨基因过氧化物酶增殖激活受体(PPAR)γC161T多态性对低出生体质量新生儿的影响。方法用横断面调查法,使用统一的调查表,对入住本院分娩的孕妇及其单胎、活胎的低出生体质量儿和健康对照新生儿进行调查,共得到有效样本268个母亲-新生儿对,并根据出生体质量和孕周进行分组,分为健康对照组和低出生体质量组,其中低出生体质量组包括早产儿组和小于胎龄儿组。使用多聚酶链-限制性片段长度多态性(PCR-RFLP)方法检测其外周血基因PPARγC161T单核苷酸多态性,分析不同基因型对其低出生体质量的影响。结果 1.根据双变量Logistic回归分析得出低出生体质量与父母的受教育水平、职业、家庭居住环境和氛围及维生素的补充相关,相关系数分别为-0.434、-0.337、-0.343、-0.269、-0.691、0.296、0.235。2.PPARγC161T多态性CC型、CT型与TT型基因频率分布在健康对照组、小于胎龄儿组和早产儿组分别为53.33%、14.44%、21.11%,75.00%、18.18%、6.82%和76.67%、20.00%、3.33%,早产儿组和小于胎龄儿组比较均无统计学差异,但与健康对照组比较均有统计学差异。携带C等位基因增加低出生体质量的风险,低出生体质量组携带CC基因型风险与健康出生体质量儿的OR值分别为1.938(95%CI:1.001～3.750,P=0.002)、2.122(95%CI:1.091～4.127,P=0.000)。结论低出生体质量是环境和遗传因素共同作用的结果,PPARγC161T多态性与低出生体质量有关,C等位基因可能是低出生体质量的遗传易感基因。Objective To study the relationship of the peroxisome proliferator-activated receptor（PPAR）γ C161T gene polymorphism and low birth weight infants. Methods A cross-sectional survey was performed by using a unified questionnaire to investigate and control a total effective sample of 268 mothers-newborns from the Affiliated Hospital of Guilin Medical College,including pregnant women and their singleton pregnancies,normal neonates,low birth weight infants.According to their birth weight and gestational age,the neonates were divided into healthy control group and low birth weight group（including preterm infants group and small for gestational age group）.The polymerase chain-restriction fragment length polymorphism（PCR-RFLP） method was used to detect single nucleotide polymorphism analysis of diffe-rent genotypes of the blood gene PPARγ C161T of low birth weight. Results 1.According to bivariate Logistic regression analysis,low birth weight and parents′ educational level,occupation,living environment and family atmosphere and vitamin supplement were correlated,and the correlation coefficients were-0.434,-0.337,-0.343,-0.269,-0.691,0.296,and 0.235,respectively.2.PPARγ C161T polymorphism genotype distribution of CC,CT and TT and type of gene frequency distribution in normal and low birth weight group of preterm infants and small for gestational age group were 53.33%,14.44%,21.11%,75.00 %,18.18%,6.82%,76.67%,20.00%,and 3.33%,respectively.Small for gestational age and preterm infants group had no significant difference,but all compared with the health control group were significantly different.Carrying the C allele increased the risk of low birth weight.Low birth weight infants carrying the risk of CC genotype,compared with normal birth weight children,their OR=1.938（95%CI：1.001-3.75,P=0.002）,2.122（95%CI：1.091-4.127,P=0.000）. Conclusions Low birth weight infants are affected by all of environmental and genetic factors.PPARγ C161T gene polymorphism is related to low birth weight,and C allele ma

关 键 词：过氧化物酶增殖激活受体γC161T 多聚酶链-限制性片段长度多态性 低出生体质量儿 婴儿 新生 

分 类 号：R722[医药卫生—儿科]