羟基喜树碱微透析回收率的体外研究  

Study on in vitro microdialysis recovery of hydroxycamptothecine

作　　者：谢毅[1] 凌家俊[1] 古锦辉[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510006

出　　处：《中国中药杂志》2012年第10期1491-1493,共3页China Journal of Chinese Materia Medica

基　　金：广东省科学技术厅重点引导项目(2010B030700035)

摘　　要：目的:建立羟基喜树碱微透析探针回收率的测定方法,考察影响回收率的因素,为体内微透析研究提供依据。方法:采用浓度差法(增量法、减量法)测定羟基喜树碱探针回收率,考察流速、浓度、温度对回收率的影响,并考察回收率的稳定性。结果:增量法和减量法测得的回收率一致;回收率与探针周围媒介浓度无关,稳定性良好,随温度升高而升高。结论:微透析技术可用于羟基喜树碱药动学研究,反透析法可用于羟基喜树碱体内微透析回收率的体内测定。Objective： To establish the mothod to dectect the microdialysis recovery of HCPT and to investigate the influencing factors, thus to supply experimental basis for in-vivo microdialysis of HCPT. Method： The in vitro recovery of HCPT was detected by concentration difference method （ increment method and decrement method ）. The influence of flow rates, medium concentration and temperature on the HCPT recovery and the stability were studied. Result： The recovery detected by increment method was the same as by decrement method. The recovery was independent of HCPT concentrations in the medium. The Hydroxycamptothecine recovery had good stability and increased as the temperature rose. Conclusion： Microdialysis sampling can be used for the pharmacokinetic study of HCPT. Retrodialysis can be used for the determination of the HCPT in vivo recovery.

关 键 词：羟基喜树碱 微透析 回收率 

分 类 号：R285[医药卫生—中药学]