卵泡液中白细胞介素12、18及自然杀伤细胞活化与体外受精-胚胎移植妊娠结局关系探讨  

Correlation between interleukin-12,interleukin-18,NK cells in follicular fluid and outcomes of in vitro fertilization embryo transfer

作　　者：王天任[1] 王秀霞[1] 何丽霞[1] 王秀华 

机构地区：[1]中国医科大学附属盛京医院妇产科,辽宁沈阳110001 [2]科尔沁区妇婴医院妇产科,内蒙古通辽028000

出　　处：《中国实用妇科与产科杂志》2012年第5期375-377,共3页Chinese Journal of Practical Gynecology and Obstetrics

基　　金：辽宁省教育厅科学基金(30371376)

摘　　要：目的探讨卵泡液中白细胞介素12(IL-12)和白细胞介素18(IL-18)与CD56+NK细胞及活化的CD56+NK(CD56+CD69+NK)细胞之间的关系,及其对体外受精-胚胎移植(IVF-ET)妊娠结局的影响。方法 2007年7月至2009年4月对中国医科大学盛京医院首次接受IVF助孕治疗的53例患者,应用流式细胞学技术测定成熟卵泡液NK细胞及活化NK细胞比例,应用酶联免疫方法测定成熟卵泡液中IL-12和IL-18的质量浓度。结果 53例患者移植5周后临床妊娠24例(妊娠组),未妊娠29例(未妊娠组)。未妊娠组中IL-12(1.74±1.81)ng/L、IL-18(134.62±159.71)ng/L明显高于妊娠组(0.67±0.91)ng/L、(78.6±70.18)ng/L,P均<0.05;未妊娠组中CD56+NK细胞数占总淋巴细胞数的百分比(20.06±5.08)%、占CD56+NK细胞的百分比(4.58±2.22)%明显高于妊娠组(14.64±3.20)%、(2.39±1.02)%,P均<0.05;IL-12与CD56+NK细胞比例的相关系数r=0.49,P<0.05,两者存在显著的正相关。IL-18与CD56+NK细胞比例的相关系数r=0.54,P<0.05,两者存在显著的正相关。IL-18与活化的CD56+NK比例的相关系数r=0.57,P<0.05,两者存在显著的正相关。结论卵泡液中IL-12、IL-18和CD56+NK细胞及活化的CD56+NK细胞的比例在接受IVF-ET助孕治疗的非妊娠妇女中明显升高,与妊娠结局呈负相关。Objective To investigate the correlation between interleukin-12 （ IL-12 ）, interleukin-18 （ IL-18 ）, NK cells （ CD56^＋ NK cells）, activated NK cells（ CD56^＋ CD69^＋ NK cells） in follicular fluid and the outcomes of IVF-ET. Methods Patients who accepted IVF-ET treatment for the first time between Jul. 2007 and APS 2009 in China Medical University Shengjing Hospital Reproductive Medicine Center were included. Triple color flow cytometry was Used to analyze the proportion of CD56^＋ NK cells and activated CD56^＋ NK cells in mature follicular fluid. Enzyme-linked immunosorbent assay （ELISA）was used to analyze the concentration of IL-2, IL-12 and IL-18 in follicular fluid. Results Patients without successful IVF-ET outcome had concentration of increased IL-12（ 1.74±1.81 ） ng/L and IL-18 （ 134. 62±159.71 ） ng/L, P 〈 0. 05. Compared with patients who achieved clinical pregnancy IL-12 （0. 67±0. 91 ） ng/L and IL-18 （ 78.6±70. 18 ） ng/L. The proportion of CD56^＋ NK cells and activated CD56^＋ NK cells in mature follicular fluid of women who got pregnancy by IVF treatment was（14. 64±3.20） % and （2. 39±1.02） % respectively, while the proportion of the women who didn＇t get pregnancy was （20. 06±5.08 ） % and（4. 58±2. 22） % respectively, which was significantly higher than the pregnant group（P 〈0. 05）. The correlation coefficient of IL-12 and CD56^＋ NK cells is r =0. 49,P 〈0. 05, which has positive statistical significance. The correlation coefficient of IL-18 and CD56^＋ NK cells is r = 0. 54, P 〈 0. 05, which has positive statistical significance. The correlation coefficient of IL-18 and CD56^＋ CD69^＋ NK cells is r = 0. 57, P 〈 0. 05, which has positive statistical significance. Conclusion Women received IVF treatment with higher concentrations of IL-12, IL-18 and higher proportion of CD56^＋ NK cells and activated CD56^＋ NK ceils in mature follicular fluid are more difficult to get pregnancy.

关 键 词：体外受精-胚胎移植 卵泡液 白细胞介素12 白细胞介素18 自然杀伤细胞 

分 类 号：R321-33[医药卫生—人体解剖和组织胚胎学]