多重耐药菌中β-内酰胺酶基因监测  

Surveillance on beta-lactamase genes in multi-drug resistance bacteria

作　　者：何晓锋[1] 刘芳[1] 曹晋桂[1] 焦力群[1] 张虎[1] 吴镝[1] 马文杰[1] 

机构地区：[1]空军总医院感染控制科,北京100142

出　　处：《现代预防医学》2012年第10期2546-2547,2550,共3页Modern Preventive Medicine

摘　　要：目的了解多重耐药的铜绿假单胞菌和鲍曼不动杆菌中β-内酰胺酶基因的分布情况。方法采用聚合酶联链反应(PCR)检测78株多重耐药的铜绿假单胞菌和鲍曼不动杆菌中的TEM、SHV和CTX—M等3种β-内酰胺酶基因,并进行DNA测序。结果 50株铜绿假单胞菌中37株TEM基因阳性(74.0%),未检出SHV和CTX—M基因,28株鲍曼不动杆菌中25株TEM基因阳性(89.3%),2株SHV阳性(7.1%),未检出CTX—M基因。结论多重耐药的铜绿假单胞菌和鲍曼不动杆菌中的β-内酰胺酶基因类型以TEM型较为流行。OBJECTIVE To investigate the beta-lactamase genes in multi-drug resistance（MDR）Pseudomonas aeruginosa and Acinetobacter baumannii.METHODS Polymerase chain reaction（PCR）was used to detect three kinds of β-lactamase genes TEM,SHV,and CTX-M in 78 multi-drug resistance strains,including Pseudomonas aeruginosa and Acinetobacter baumannii.The PCR products were sequenced by DNA sequence.RESULTS Among 50 multi-drug resistance strains in Pseudomonas aeruginosa,there were 37 strains TEM gene-positive（74.0%）,SHV and CTX-M gene were not detected.Among 28 multi-drug resistance strains in Acinetobacter baumannii.TEM gene were positive in 25 strains（89.3%）,SHV gene were positive in 2 strains（7.1%）,and CTX-M gene was not detected.CONCLUSION The multi-drug resistance strains in Pseudomonas aeruginosa and Acinetobacter baumannii have carried out various kinds of beta-lactamase resistance gene,however TEM genetype are prevalent.

关 键 词：多重耐药 Β-内酰胺酶 基因 

分 类 号：R378[医药卫生—病原生物学]