μ阿片受体基因A118G多态性对患者电刺激痛觉敏感性的影响  

Effects of A118G polymorphism in μ opioid receptor gene on pain sensitivity to electric stimulation

作　　者：王中玉[1] 张豪勇[1] 常琰子[1] 袁静静[1] 李治松[1] 张卫[1] 阚全程[2] 

机构地区：[1]郑州大学第一附属医院麻醉科,450052 [2]郑州大学第一附属医院临床药理基地河南省高等学校临床医学重点学科开放实验室,450052

出　　处：《临床麻醉学杂志》2012年第4期357-359,共3页Journal of Clinical Anesthesiology

摘　　要：目的探讨μ阿片受体基因A118G(OPRM1A118G)多态性对手术患者电刺激痛觉敏感性的影响。方法择期拟行腹部手术患者162例,ASAⅠ或Ⅱ级,男72例,女90例。采用电刺激测定患者痛阈和耐痛阈。采用聚合酶链反应-限制性片段长度多态性技术(PCR-RFLP)进行OPRM1A118G多态性位点的检测。结果共三种基因型:野生型纯合子(A/A)79例(男31例,女48例),突变型杂合子(A/G)64例(男34例,女30例),突变型纯合子(G/G)19例(男7例,女12例)。G等位基因频率为31.5%(男性33.3%,女性30.0%)。不同基因型患者年龄、BMI、痛阈和耐痛阈差异无统计学意义。不同基因型男性患者痛阈和耐痛阈均明显高于女性(P<0.05或P<0.01)。结论 OPRM1A118G等位基因突变对患者电刺激的痛阈和耐痛阈无明显影响。Objective To investigate the effects of A118G polymorphism in μ-opioid receptor gene on pain sensitivity to electric stimulation in patients undergoing operation.Methods A total of 162 patients（ASAⅠorⅡ,72 male and 90 female） scheduled for abdominal surgery were included in the study.Pain threshold and pain tolerance threshold were measured using electrical stimulation.Genotypes were detected by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） for A118G polymorphism.Results Genotyping analysis revealed that the genotype distribution of A118G polymorphism was 79/162 wild-type homozygous（A/A,31 male and 48 female）,64/162 mutant heterozygous（A/G,34 male and 30 female）,and 19/162 mutant homozygous（G/G,7 male and 12 female）.The G allele frequency was 31.5%（male 33.3%,female 30.0%）.There were no differences in age,BMI,pain threshold and pain tolerance among the 3 genotype groups;.the pain threshold and pain tolerance were significantly higher in males than females（P〈0.05 or P〈0.01）.Conclusion The A118G allele gene has no effects on pain threshold and pain tolerance threshold to electric stimulation in patients undergoing operation.

关 键 词：Μ阿片受体 基因多态性 单核苷酸 电刺激 痛阈 耐痛阈 

分 类 号：R614[医药卫生—麻醉学]