吗啡对人胃癌MGC-803细胞caspase-3表达的影响  

The effects of morphine on caspase-3 expression in human gastric carcinoma MGC-803 cells

作　　者：梁羽冰[1] 覃怡[1] 谢玉波[1] 

机构地区：[1]广西医科大学第一附属医院麻醉科,南宁市530021

出　　处：《临床麻醉学杂志》2012年第4期395-396,共2页Journal of Clinical Anesthesiology

基　　金：国家自然科学基金(81160289);广西科学研究与技术开发计划项目(桂科攻1140003A-12);广西教育厅科研资助项目(201012MS036)

摘　　要：目的观察吗啡对人胃癌MGC-803细胞凋亡及caspase-3表达的影响。方法胃癌MGC-803细胞分为两组:对照组和吗啡组。对照组不加任何药物,吗啡组将吗啡加入细胞培养液中使吗啡终浓度为0.1μmol/L。孵育24h后应用流式细胞仪检测细胞凋亡情况,并应用半定量逆转录-聚合酶链反应(RT-PCR)和Western blot技术检测胃癌MGC-803细胞中caspase-3基因mRNA和蛋白的表达情况。结果吗啡组胃癌细胞凋亡率为(17.20±2.95)%,明显高于对照组的(11.40±2.86)%(P<0.05)。RT-PCR和Western blot结果显示,吗啡组胃癌MGC-803细胞中caspase-3mRNA和蛋白表达增加。结论 0.1μmol/L吗啡通过增加caspase-3的表达而促进胃癌细胞的凋亡。Objective To explore the effects of morphine on the apoptosis of human gastric carcinoma MGC-803 cells and the expression of caspase-3.Methods MGC-803 cells were divided into two groups.The cells were incubated with or without 0.1 μmol/L morphine.The apoptosis was assessed by flow cytometry after 24 h.The expression of caspase-3 mRNA and proteins were detected by semi-quantitative reverse transcription polymerase chain reaction（RT-PCR） and Western blot.Results The apoptotic rate of MGC-803 cells in the morphine group was higher than that in the control group.Caspase-3 mRNA and protein were up-regulated by morphine treatment.Conclusion 0.1 μmol/L morphine may promote the apoptosis of gastric cancer cells by up-regulating caspase-3 in vitro.

关 键 词：吗啡 胃癌 凋亡 CASPASE-3 

分 类 号：R614[医药卫生—麻醉学]