血红素加氧酶-1对急性肺损伤大鼠肺泡Ⅱ型上皮细胞的保护作用  

Protective effect of heme oxygenase-1 on alveolar epithelial type Ⅱ cell in acute lung injury rats

作　　者：谭泽龙[1,2] 陈淼[1] 曹海军[1] 钱明江[1] 王洪敏[1] 谢海辉[1] 

机构地区：[1]遵义医学院附属医院ICU,563003 [2]山东省泰安市中心医院麻醉科

出　　处：《临床麻醉学杂志》2012年第4期406-408,共3页Journal of Clinical Anesthesiology

基　　金：贵州省教育厅自然科学类科研项目(黔教科2007042)

摘　　要：目的探讨血红素加氧酶-1(HO-1)对急性肺损伤(ALI)大鼠肺泡Ⅱ型上皮细胞(AEC-Ⅱ)的保护作用。方法雄性SD大鼠32只,随机均分为四组:正常对照组(C组),油酸损伤组(OA组),阿司匹林预处理组(AS组)和锌卟啉IX预处理组(IX组)。测定动脉血气,支气管肺泡灌洗液(BALF)和血清中肺表面活性物质相关蛋白A(SP-A)含量,肺组织湿/干重比(W/D);免疫组化法测肺组织HO-1蛋白表达,电镜下观察AEC-Ⅱ超微结构的变化。结果与C组比较,其余各组W/D、HO-1蛋白表达明显升高(P<0.01)。与OA组比较,AS组W/D明显下降(P<0.01),HO-1蛋白表达明显升高(P<0.01);IX组W/D明显升高(P<0.05),HO-1蛋白表达明显下降(P<0.01)。电镜显示OA组可见AEC-Ⅱ相对不规则,细胞变性甚至崩解,细胞表面微绒毛减少,胞浆内板层小体排空,部分排入肺泡腔。AS组较OA组轻;IX组较OA组重。结论 HO-1的高表达具有明显的保护AEC-Ⅱ的作用,进而对急性肺损伤有防治作用。Objective To study the protective effect of heme oxygenase-1（HO-1） on rat’s alveolar epithelial type Ⅱ cells in acute lung injury（ALI）.Methods Thirty-two male SD rats weighing 180-200 g were randomly divided into 4 groups（n=8 each）：group C（control group）;group OA（injury group）;group AS（aspirin pretreatment group）;group IX（zinc porphyrin IX pretreatment group）.The arterial blood gas,the SP-A content of bronchoalveolar lavage fluid（BALF） and serum,and the wet/dry（W/D）weight ratio of lung tissue were examined.The expression of HO-1 protein in the lung tissue and the ultrastructural changes of AEC-Ⅱ were also observed.Results There was significant difference in the wet/dry（W/D）weight ratio and the expression of HO-1 protein in all of the groups except group C（P〈0.01）.Compared with group OA,the wet/dry（W/D）weight ratio decreased and the expression of HO-1 protein increased in group AS,but the wet/dry（W/D）weight ratio increased and the expression of HO-1 protein decreased in group IX.Relatively irregular AEC-Ⅱ,cell degeneration or disintegration,reduction of cell surface microvilli,intracytoplasmic lamellar body emptying,and part into the alveolar space were found by electron microscopy,with a severity sequence of group AS〈OA〈IX.Conclusion The high expression of HO-1 has an obviously protective effect on acute lung injury by acting on AEC-Ⅱcells.

关 键 词：血红素加氧酶-1 急性肺损伤 肺泡Ⅱ型上皮细胞 大鼠 

分 类 号：R563.8[医药卫生—呼吸系统]