BDNF基因修饰的NSCs移植对大鼠脊髓损伤后轴突再生及后肢运动功能的影响  

Effects of neural stem cell modified by BDNF transplantation on the axonal regeneration and function of hind limbs in rats after spinal cord injury

作　　者：张国庆[1] 樊立华 徐红梅[1] 孙奎兴[3] 

机构地区：[1]青岛大学医学院附属医院,山东青岛266100 [2]东营市人民医院 [3]青岛大学医学院附属医院海阳分院

出　　处：《山东医药》2012年第15期48-50,共3页Shandong Medical Journal

基　　金：青岛市科技发展计划资助项目(10-3-1-7-1-jch)

摘　　要：目的探讨脑源性神经营养因子(BDNF)基因修饰的神经干细胞(NSC-BDNF)移植对脊髓损伤(SCI)的可行性及效果。方法将120只SCI大鼠随机分为四组各30只,假手术组(Sham组)仅做椎板切开术,伤后3 d损伤组(SCI组)脊髓内注射生理盐水溶液5μl,NSC组髓内注射移植细胞密度为2×105/μl的NSCs悬液5μl,NSC-BDNF组髓内注射NSC-BDNF悬液5μl。采用免疫组织化法检测1、3、7、14、28 d脊髓组织BDNF、神经丝蛋白(NF200)表达;行为学评分法判断后肢运动功能恢复情况。结果 NSC-BDNF组BDNF水平明显高于其他三组,且随着时间延长而增高(P<0.05或0.01);NSC-BDNF组NF200水平、运动功能评分明显高于SCI组、NSC组,低于Sham组,且随着时间延长而增高和降低(P<0.05或0.01)。结论 NSC-BDNF在损伤脊髓内可存活、迁移,并参与SCI神经轴突的结构重建,从而促进后肢运动功能恢复。Objective To investigate the effects of neural stem cell （NSC） modified by brain derived neurophic factor （BDNF） transplantation on the axonal regeneration and function of hind limbs in rats after spinal cord injury（SCI）. Meth- ods Total of 120 SCI rats were divided randomly into 4 groups with 30 in each, the Sham groupreceived laminotomy only, the 3 d after SCI group was given 5 μL normal saline by intraspinal injection, the NSC group was given 5μL cell suspension of NSC（2×10^5/μL）, the NSC-BDNF group was given 5 μL cell suspension of NSC-BDNF. After 1,3,7,14,28 d, the BDNF and NF200 were detected by immunochemistry, hind limb function of the rats was evaluated using BBB open field locomotion rating score. Results The level of BDNF in NSC-BDNF group was significantly higher than that in the other three groups（ P 〈 0.05,0.01 ） in time-dependent manner. The level of NF200 in NSC -BDNF group was significantly higher than that in the other three groups （P 〈 0.05 ）, and the BBB score was distinctly higher than that in the SCI and NSC group, was lower than that in Sham group （ P 〈 0.05,0. 01 ） in time-dependent manner. Conclusion Transplantation of NSCs modified by BDNF can survive in the injured site of spinal cord, can promote the axonal reconstruction and recovery of the function of the hind limb in rats after SCI.

关 键 词：脊髓损伤 脑源性神经营养因子 神经干细胞 轴突再生 后肢功能 

分 类 号：R651.2[医药卫生—外科学]