贵州特有爬竹的组织培养研究  

Tissue Culture of Particular and Critically Endangered Bamboo Species—Drepanostachyum scandens in Guizhou Province

作　　者：孟文艺[1] 苟光前[2] 何林健[1] 马玉栋[2] 

机构地区：[1]贵州大学林学院,贵州贵阳550025 [2]贵州大学竹类研究所,贵州贵阳550025

出　　处：《山地农业生物学报》2012年第2期116-118,共3页Journal of Mountain Agriculture and Biology

基　　金：贵阳市农业攻关项目[(2008)筑科农合同字第43号]

摘　　要：以无菌苗率和优良芽率为主要指标,通过对比不同基本培养基和6-BA浓度的试验,以探索爬竹侧芽适宜的消毒方法和诱导培养基。结果表明,爬竹侧芽适宜的消毒方法为:75%酒精处理1 min后再用0.1%升汞处理8～10 min,无菌苗率可达75%左右;采用3/5MS基本培养基时优良芽率最高,显著优于MS、3/4 MS及1/4 MS;在不同激素组合下,6-BA浓度为2 mg/L时优良芽率最高,当6-BA浓度高于2 mg/L时,优良芽率呈显著性减小。The method for explant disinfection and the induction medium for lateral bud of Drepanostachyum scandens were optimized in the present work.The results showed that the optimal explant disinfection was 75% alcohol for 1min,followed by 0.1% HgCl2 for 6-8min,by which around 75% of fungi-free explants might be gained.Among the tested basic medium,3/5 MS gave the best effect for vigorous bud induction.6-BA contributed highly to bud induction,and 2mg/L was the best concentration for strong bud induction.

关 键 词：爬竹 组织培养 萌芽率 优良芽率 

分 类 号：S722.37[农业科学—林木遗传育种]