干扰素体外释放酶联免疫法在结核潜伏感染诊断中的价值  

Evaluation of the value of Quantitative Diagnostic Kit for Mycobacterium tuberculosis-IFN-γ release assay in screaming latent Mtb infection diagnosis

作　　者：翁绳凤[1,2] 刘佳文[2] 康丽军[2] 刘威[2] 刘宏蕴 李宝玲[2] 朱新颖[2] 何燕[1] 

机构地区：[1]首都医科大学公共卫生与家庭医学院,北京100069 [2]北京老年医院检验科,北京100095

出　　处：《临床肺科杂志》2012年第6期1062-1064,共3页Journal of Clinical Pulmonary Medicine

摘　　要：目的探讨干扰素体外释放酶联免疫法(TB-IGRA)在诊断诊断结核分枝杆菌潜伏感染的价值。方法分枝杆菌相关γ-干扰素检测试剂盒(WT-IGRA)检测140例健康者IFN-γ分泌水平,并对其进行结核菌素皮试及采用Cellestis Limited生产的QuantiFERON-TB试剂盒进行IFN-γ平行检测。结果 140例健康者中,WT试剂和QFT试剂阳性率分别是25.7%(36/140)、21.4%(30/140),PPD3+～4+为14.3%(20/140)。两试剂阳性符合率为81.3%,阴性符合率为88.9%,总符合率86.7%。28例密接者中,PPD3+～4+为39.3%(11/28),WT试剂和QFT试剂阳性率分别是46.4%(13/28)和67.8%(19/28)。结论 TB-IGRA在诊断结核分枝杆菌潜伏感染的价值优于PPD皮试,可以用于潜伏感染者的筛查。Objective To investigate the clinical value of the Quantitative Diagnostic Kit for Mycobacterium tuberculosis-IFN-γrelease assay（TB-IGRA） in diagnosing latent Mycobaterium tuberculosis infection.Methods 140 subjects were analyzed;antigen specific-IFN response were determined by using regents from two suppliers,Quantitative Diagnostic Kit for Mycobacterium tuberculosis-IFN-γ release assay（WT-IGRA） and QuantiFERON-TB GOLD in tube（QFT-GIT,Cellestis Limited,Australia）.Subjects received PPD skin test as well.Results Among 140 subjects,the positive rates of TB-IGRA with WT and QFT-G were 25.7（36/140）,21.4%（30/140）,PPD3＋～4＋ was 14.3%（20/140）.The positive consistency of two kits was 81.3%,negative consistency was 88.9%,total consistency was 86.7%.Among 28 close contacts,their PPD3＋～4＋was 39.3%（11/28）,WT and QFT positive rates were 46.4%（13/28） and 67.8%（19/28）.Conclusions The diagnostic value of latent Mtb infection of TB-IGRA is higher than that of PPD method,TB-IGRA can be used as a screening method for latent Mtb infection patients.

关 键 词：结核分枝杆菌潜伏感染 干扰素释放试验 结核菌素皮试 

分 类 号：R52[医药卫生—内科学]