应用转座子Tn5对生防菌P-936进行遗传改良的研究  

Studies on Genetic Improvement of Biological Control Bacterium P-936 by Transposon Tn5

作　　者：李湘民[1] 黄瑞荣[1] 华菊玲[1] 罗任华[1] 刘平安 

机构地区：[1]江西省农业科学院植物保护研究所,江西南昌330200 [2]江西省进贤县农业局,江西进贤330400

出　　处：《江西农业学报》2012年第4期102-104,共3页Acta Agriculturae Jiangxi

基　　金：江西省科技厅农业攻关项目(2004-2006)

摘　　要：以广谱性拮抗细菌荧光假单胞菌P-936为受体菌,大肠杆菌Escherichia coli S17-1 SM10/pSUP2021::Tn5为供体菌,通过结合转移技术,将转座子Tn5从供体菌中引入到受体菌P-936的基因组DNA中,共获得3011个Tn5插入突变体。在离体测定中,有4个突变体对稻瘟病菌的拮抗作用明显增强,有1个突变体完全丧失了拮抗作用;2个突变体对番茄青枯病菌的拮抗作用明显增强。通过盆栽和田间小区试验,突变体T-1207在连续3次防治后,对稻瘟病的防治效果达到61.97%,而野生菌株P-936的防治效果为51.55%,表现了一定的增效作用。Using a wide-spectrum antagonistic bacterium Pseudomonas fluorescens P-936 as acceptor,and Escherichia coli S17-1 SM10/pSUP2021：Tn5 as supplier,the transposon Tn5 from the supplier was transferred into the genomic DNA of P-936 by combination-transfer technique.Total 3011 mutants with the transferred Tn5 were obtained.In the isolated test,the antagonistic effects of 4 mutants against rice blast were obviously enhanced,while one mutant wholly lost its antagonistic effect;the antagonistic effects of two mutants were markedly enhanced against tomato blight.In the potted plant test and field plot experiments,the control efficiency of mutant T-1207 against rice blast reached 61.97%,while that of wild P-936 was 51.55% after continuous control for 3 times.

关 键 词：转座子 生防菌 遗传改良 

分 类 号：S476.19[农业科学—农业昆虫与害虫防治]