胎膜早破孕妇胎膜组织核转录因子和COX-2的表达及临床意义  

Expressions and clinical significance of nuclear factor kappa B and cyclooxygenase-2 in premature rupture of membrane

作　　者：周甜甜[1] 汪萍 孔祥 

机构地区：[1]江苏省连云港市第一人民医院妇产科,江苏连云港222000 [2]扬州大学临床医学院妇产科,江苏扬州225001

出　　处：《实用临床医药杂志》2012年第7期49-52,共4页Journal of Clinical Medicine in Practice

基　　金：江苏省扬州市社会发展项目(YZS2007003)

摘　　要：目的通过检测胎膜组织中核转录因子(NF-κB)和环氧化酶-2(COX-2)蛋白的表达,分析其在胎膜早破(PROM)发病中的作用及相关性。方法采用HE染色镜检胎膜病理切片,将病例分为非绒毛膜羊膜炎组和绒毛膜羊膜炎组;采用免疫组化SP法检测32例PROM和20例正常孕妇胎膜组织中NF-κB和COX-2的表达。结果所有PROM胎膜标本,正常组胎膜感染占10.00%,绒毛膜羊膜炎者占46.88%;3组PROM组孕妇胎膜组织中NF-κB、COX-2表达明显高于对照组孕妇(P<0.05);绒毛膜羊膜炎孕妇组胎膜NF-κB、COX-2表达明显高于非绒毛膜羊膜炎孕妇组(P<0.05);在PROM胎膜组织中NF-κB/p65阳性表达与COX-2阳性表达成明显正相关关系(P<0.01)。结论 NF-κB、COX-2参与了PROM的发生过程。COX-2基因的表达可能受NF-κB的调控。NF-κB活化与PROM发生关系密切。Objective To detect the expressions of nuclear factor kappa B（NF-κB） and cyclooxygenase-2（COX-2） and analyze their roles and correlation in premature rupture of membrane（PROM）.Methods Membranes were immediately collected after labor for pathological examination of the fetal membrane by HE staining.The patients were divided into non-chorioamnionitis group and chorioamnionitis group.Using immunohistochemical SP method to assess the expressions of NF-κB and COX-2 in the specimens of 32 PROM patients and 20 normal puerperas.Results After HE staining,the infection rate was 46.88%（15/32） in the PROM group and 10.00%（2/20） in the normal group.Positive NF-κB and COX-2 were significantly higher in fetal membranes in the PROM group than in the normal group（P0.05）.The expressions of NF-κB and COX-2 in the chorioamnionitis group were higher than those in the non-chorioamnionitis group（P0.05）.There was a significant positive correlation between the expressions of NF-κB and COX-2（P0.01）.Conclusion NFκB and COX-2 play an important role in the pathogenesis of PROM.The expressions of COX-2 mar be regulated by NF-κB.The activation of NF-κB has a close relationship with PROM.

关 键 词：胎膜早破 核转录因子(NF-κB) 环氧化酶-2(COX-2) 免疫组化 

分 类 号：R714.433[医药卫生—妇产科学]