快生型大豆根瘤菌基因库的构建  

Construction of Total DNA Gene Library of Rhizobium Fredii

作　　者：沈辉[1] 张忠明[1] 曹燕珍[1] 

机构地区：[1]武汉华中农业大学土化系,武汉430070

出　　处：《中国微生态学杂志》1990年第4期51-56,共6页Chinese Journal of Microecology

摘　　要：本文报道了用柯斯质粒(cosmid)pLAFRI 作为载体,通过 EcoRI 部分酶切快生型大豆根瘤菌的全部 DNA,获得“目的”DNA 片段,用大肠杆菌 ED8767作为受体菌株,我们构建了一株快生型大豆根瘤菌——B52菌株的基因库。插入的基因片段大小为20～30kb,所获得的抗性菌落数为3.9×10~4,其中外源基因插入频率为70%,重组子数超过“理论”值,达到了希望的建库要求。The total DNA gene bank of R.fredii strain B_(52) was constructed by using cosmid pLAFRI as gene vector.The total DNA was partially digested with restriction edonuclease EcoRI and size fractioned.The range of inserted fragment is from 20 to 30 kilobase.E.coli ED8767 was used as the recipient strain and 3.9x10^4 recombinant colonies were isolated and 70 percent of them contained foreign DNA fragment, which was enough for a “complete” gene library of R.fredii.

关 键 词：大豆根瘤菌 基因库 

分 类 号：Q939.114[生物学—微生物学]