间歇低氧对大鼠交感神经兴奋性的影响及抗氧化剂的干预作用  

Influence of chronic intermittent hypoxia on sympathetic nerve activity in rats and effect of the antioxidant Tempol

作　　者：林慧[1] 陈宝元[1] 姜秋芳[1] 孔铖英[1] 张静[1] 郑立文[1] 

机构地区：[1]天津医科大学总医院呼吸科,300052

出　　处：《中华结核和呼吸杂志》2012年第5期345-348,共4页Chinese Journal of Tuberculosis and Respiratory Diseases

基　　金：国家自然科学基金（30770934,81100060）

摘　　要：目的探究大鼠交感神经兴奋性在慢性间歇低氧（CIH）诱发高血压过程中的作用及交感神经兴奋性增强的机制与氧化应激的关系，观察抗氧化剂4-羟基-2，2，6，6-四甲基哌啶（Temp01）对血压的干预作用。方法48只雄性Wistar大鼠按随机数字表法分为6组，每组8只，分别为常氧对照组、间歇低氧空白对照组（低氧对照组）、Temp01预干预组（干预1组）、Tempol后干预组（干预2组）、生理盐水预对照组（对照1组）及后对照组（对照2组）。除常氧对照组外均给予间歇低氧环境，其中干预1组和干预2组分别于实验前和实验后第28天给予10％Tempol100mg·kg～·d。腹腔注射，对照1组和对照2组同时分别给予等量生理盐水腹腔注射。测定大鼠动脉收缩压、血清去甲肾上腺素、肾上腺素水平以及肾上腺组织匀浆中丙二醛水平。结果6周后，低氧对照组收缩压与干预1组和干预2组相比，差异无统计学意义。干预1组收缩压[（114-4-6）mmHg，1mmHg：0．133kPa]与常氧对照组和实验前相比无明显变化，其余各组大鼠收缩压均高于常氧对照组和实验前水平（F值为15．045，均P〈0．05）。干预1组和干预2组收缩压[（128±6）mmHg]均低于对照1组[（138±10）mmHg]和对照2组[（138±10）mmHg，均P〈0．05]，但干预2组高于干预1组（P〈0．01）。常氧对照组收缩压水平与实验前比较差异无统计学意义。低氧对照组与对照1组和对照2组相比，血清去甲肾上腺素、肾上腺素及肾上腺匀浆中丙二醛水平均无统计学意义。干预1组和干预2组去甲肾上腺素、肾上腺素及丙二醛水平均低于对照1组和对照2组（P〈0．05），但干预2组各指标仍高于常氧对照组（P〈0．05）及干预1组（P〈0．05或P〈0．01）。干预1组去甲肾上腺素、肾上腺素及丙二醛水平与常氧对照组比较无明显差异。结论CIH可能导致交感神经活性增强，Objective To study the role of sympathetic nerve activity during the development of hypertension resuhing from chronic intermittent hypoxia, and whether or not elevated sympathetic nerve activity is related to oxidative stress, and to investigate the preventive effect and possible mechanism of Tempol. Methods Forty-eight male Wistar rats were randomly divided into 6 groups of 8 each, including normoxic control group （NC）, intermittent hypoxia group （IH） and 4 treatment groups （IHTI,IHT2, IHN1, IHN2）. Among the treatment groups, IHT1 ,IHT2 groups were treated with 10% Tempol 100 mg ~ kg-1 ~ d-1 by intraperitoneal injection before exposed to IH and on day 28 after exposed to IH respectively, while IHN1 and IHN2 groups were treated with NS as controls. Result There was no statistic difference in artery systolic blood pressure （SBP） between IH group, IHN1 group [ （114 ± 6） mm Hg, 1 mm Hg = 0. 133 kPa ], andlHN2 group[（128±6） mm Hg,P〈0.05]at the end of 6th week. SBP in the all IH groups was significantly elevated compared with NC group （ P 〈 0. 05 ） and the baseline SBP （ P 〈 0. 05 ） except of the group [HT1. SBP in the 2 tempol treatment groups was lower than the NS groups[ （138 -＋ 10）mm Hg, both P 〈 0. 05 ] , while SBP of IHT2 group was higher than the IHT1 group（ P 〈 0. 01 ）. No significant changs were found in the NC group. There were no statistic difference of NE and E in plasma and MDA in adrenal gland tissued between IH groups, IHN1 group and IHN2 group. The levels in NE and E and MDA in the 2 tempol treatment groups were lower than the NS groups （ P 〈 0. 05 or P 〈 0. 01 ） , but those in the IHT2 group were higher than the NC group（P 〈0. 05 or P 〈0.01 ）and IHT1 groups（P 〈0. 05 or P 〈0. 01 ）. No significant defference were found between IHT1 group and NC group. Conclusion CIH could genetate ROS by causing oxitative stress, which results in elevated sympathetic nerve activity. This may be one of the important mechanisms for CIH-induced

关 键 词：睡眠呼吸暂停综合征 低氧血症 氧化性应激 4-羟基-2 2 6 6-四甲基哌啶 

分 类 号：R965[医药卫生—药理学]